Solubilization of PNS myelin membrane proteins by detergents

• Published in: Neuroreport Vol. 11; no. 11; pp. 2559 - 2563
• Main Authors: Sedzik, Jan, Tsukihara, Tomitake
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins, Inc 03-08-2000; Lippincott Williams and Wilkins
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; Central nervous system; Detergents - pharmacology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; myelin P0 protein; Myelin Proteins - chemistry; Myelin Proteins - drug effects; Myelin Proteins - isolation & purification; Myelin Sheath - drug effects; Myelin Sheath - metabolism; Peripheral Nerves - drug effects; Peripheral Nerves - metabolism; Sodium Chloride - pharmacology; Sodium Dodecyl Sulfate - pharmacology; Solubility - drug effects; Vertebrates: nervous system and sense organs; Membrane protein; Detergent; Investigation method; Myelin; Neuroglia; Glycoprotein; Solubilization; Micellar solution; Spinal root
• ISSN: 0959-4965; 1473-558X
• DOI: 10.1097/00001756-200008030-00042

We have applied 25 different detergents to the PNS myelin membrane. The extracts produced were analyzed regarding the total amount of protein solubilized, and which myelin glycoproteins were solubilized. The degree of protein extraction was correlated with the critical micelle concentration. For some detergents the protein extraction ability depends clearly on critical micelle concentration. These detergents are more potent if critical micelle concentration is small. The other type of extraction is independent on the critical micelle concentration, but extractability of proteins is low. The best detergents for solubilization of myelin proteins are neutral of the alkyl chain length n = 9–11, as followsdodecyl-β-D-maltopyranoside, decyl-β-D-maltopyranoside, n-dodecylsulfobetaine. We expect that these detergents will also be suitable for crystallization of P0 and PASII/PMP22 glycoproteins.