Potentiation of differential hyperthermic sensitivity of AKR leukemia and normal bone marrow cells by lidocaine or thiopental

• Published in: Cancer Vol. 54; no. 12; p. 2831
• Main Authors: Robins, H I, Dennis, W H, Martin, P A, Sondel, P M, Yatvin, M B, Steeves, R A
• Format: Journal Article
• Language: English
• Published: United States 15-12-1984
• Subjects: Animals; Bone Marrow - drug effects; Bone Marrow - physiology; Bone Marrow Cells; Cell Survival; Female; Hyperthermia, Induced; Leukemia, Experimental - pathology; Leukemia, Experimental - therapy; Lidocaine - pharmacology; Mice; Mice, Inbred Strains; Stem Cells - drug effects; Thiopental - pharmacology
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19841215)54:12<2831::AID-CNCR2820541202>3.0.CO;2-8

Previous work has utilized spleen colony formation to evaluate the fractional survival of AKR leukemia and normal bone marrow cells after in vitro heat exposure. An inherently greater sensitivity of neoplastic cells to thermal killing, as compared to normal syngeneic stem cells, has been established both at 41.8 degrees C and 42.5 degrees C. Normal bone marrow colony-forming units were assayed in lethally irradiated (750 cGy) mice. Leukemic colony-forming units were assayed in nonirradiated mice. Using this methodology, the authors demonstrated that the differential effect of hyperthermia on AKR murine leukemia and AKR bone marrow cells can be further enhanced by the addition of lidocaine or thiopental to incubation mixtures. These findings may have application to autologous bone marrow transplantation in humans.