Exercise Training Promotes Cardiac Hydrogen Sulfide Biosynthesis and Mitigates Pyroptosis to Prevent High-Fat Diet-Induced Diabetic Cardiomyopathy

• Published in: Antioxidants Vol. 8; no. 12; p. 638
• Main Authors: Kar, Sumit, Shahshahan, Hamid R, Hackfort, Bryan T, Yadav, Santosh K, Yadav, Roopali, Kambis, Tyler N, Lefer, David J, Mishra, Paras K
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-12-2019; MDPI
• Subjects: Antibodies; Antioxidants; Apoptosis; Biosynthesis; Cardiomyopathy; Catheters; Cell death; Diabetes; Diabetes mellitus (non-insulin dependent); Diet; Fitness equipment; Fitness training programs; Food; Gene expression; Glucose; Heart; High fat diet; Homocysteine; Hydrogen sulfide; Hyperglycemia; Inflammation; Insulin; Insulin resistance; Metabolic syndrome; MicroRNAs; Obesity; Physical training; Protein expression; Proteins; Pyroptosis; Running; Ventricle; St Louis Missouri; United States > US; metabolic syndrome; insulin resistance; inflammasome; cardiac remodeling
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox8120638

Obesity increases the risk of developing diabetes and subsequently, diabetic cardiomyopathy (DMCM). Reduced cardioprotective antioxidant hydrogen sulfide (H
S) and increased inflammatory cell death via pyroptosis contribute to adverse cardiac remodeling and DMCM. Although exercise training (EX) has cardioprotective effects, it is unclear whether EX mitigates obesity-induced DMCM by increasing H₂S biosynthesis and mitigating pyroptosis in the heart. C57BL6 mice were fed a high-fat diet (HFD) while undergoing treadmill EX for 20 weeks. HFD mice developed obesity, hyperglycemia, and insulin resistance, which were reduced by EX. Left ventricle pressure-volume measurement revealed that obese mice developed reduced diastolic function with preserved ejection fraction, which was improved by EX. Cardiac dysfunction was accompanied by increased cardiac pyroptosis signaling, structural remodeling, and metabolic remodeling, indicated by accumulation of lipid droplets in the heart. Notably, EX increased cardiac H₂S concentration and expression of H₂S biosynthesis enzymes. HFD-induced obesity led to features of type 2 diabetes (T2DM), and subsequently DMCM. EX during the HFD regimen prevented the development of DMCM, possibly by promoting H₂S-mediated cardioprotection and alleviating pyroptosis. This is the first report of EX modulating H₂S and pyroptotic signaling in the heart.