Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization

• Published in: Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology Vol. 13; no. 8; pp. 3129 - 3137
• Main Authors: Nayak, Rati Ranjan, Pradhan, Nilotpala, Behera, Debadhyan, Pradhan, Kshyama Madhusikta, Mishra, Srabani, Sukla, Lala Behari, Mishra, Barada Kanta
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2011; Springer Nature B.V
• Subjects: Bacteria; Characterization and Evaluation of Materials; Chemistry and Materials Science; E coli; Inorganic Chemistry; Lasers; Materials Science; Microorganisms; Nanocomposites; Nanomaterials; Nanoparticles; Nanostructure; Nanotechnology; Optical Devices; Optics; Photonics; Physical Chemistry; Research Paper; Silver; TEM; Antimicrobial activity; Silver nanoparticle
• ISSN: 1388-0764; 1572-896X
• DOI: 10.1007/s11051-010-0208-8

An eco-friendly microbial method for synthesis of silver colloid solution with antimicrobial activity is developed using a fungal strain of
Penicillium purpurogenum
NPMF. It is observed that increase in concentration of AgNO
3
increases the formation of silver nanoparticle. At 5 mM concentration highly populated polydispersed nanoparticles form. Furthermore, change in pH of the reaction mixture leads to change in shape and size of silver nanoparticles. At lower pH two peaks are observed in the absorption spectra showing polydispersity of nanoparticles. However, highly monodispersed spherical nanoparticles of 8–10 nm size form with 1 mM AgNO
3
concentration at pH 8. Antimicrobial activity of nanoparticles is demonstrated against pathogenic gram negative bacteria like
Escherichia coli
and
Pseudomonas aeruginosa
, and gram positive bacteria like
Staphylococcus aureus.
The antimicrobial activity of silver nanoparticles obtained at different initial pH show strong dependence on the surface area and shape of the nanoparticles.