Persistence of Parvovirus B19 in liver from transplanted patients with acute liver failure

• Published in: Future microbiology Vol. 15; no. 5; pp. 307 - 317
• Main Authors: Alves, Arthur Dr, Melgaço, Juliana G, Cássia Nc Garcia, Rita de, Raposo, Jessica V, de Paula, Vanessa S, Araújo, Cristina Cv, Pinto, Marcelo A, Amado, Luciane A
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01-03-2020
• Subjects: acute liver failure; Antibodies; Biopsy; Blood; Deoxyribonucleic acid; diagnostic; DNA; Etiology; Gene expression; Hepatitis; Herpes viruses; Immunoglobulin G; Infections; liver disease; Liver diseases; Liver failure; Liver transplantation; Medical diagnosis; Metabolic disorders; Parvovirus B19; Parvoviruses; Patients; real-time PCR; Serological tests; Serology; Transcription; Transplants & implants; Brazil; United States > US; Rio de Janeiro Brazil; diagnostic; real-time PCR; liver disease; acute liver failure; Parvovirus B19
• ISSN: 1746-0913; 1746-0921
• DOI: 10.2217/fmb-2019-0224

In this study, we investigated the presence of B19V in liver tissues from patients with acute liver failure (ALF) and evaluated the viral activity in infected liver.
Serum and liver samples from 30 patients who underwent liver transplantation for ALF were investigated for B19V infection by real-time PCR, serological tests and examination of B19V mRNA (transcript) expression in the liver.
The serum and liver samples from seven patients were B19V DNA positive (10
–10
copies/ml). Most of them presented detectable anti-B19V IgG, indicating persistent infection. B19V mRNA was detected in all patients, demonstrating intra-hepatic replication.
B19V infection of the liver during the course of non-A-E ALF suggested a role of B19V, which produced the worst outcome in co-infected patients and in patients with cryptogenic ALF, in liver damage.