Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

• Published in: International ophthalmology Vol. 39; no. 5; pp. 1123 - 1135
• Main Authors: Claros-Chacaltana, Flor Diana Yokoay, Aldrovani, Marcela, Kobashigawa, Karina Kamachi, Padua, Ivan Ricardo Martinez, Valdetaro, Gisele Pereira, de Barros Sobrinho, Alexandre Augusto Franchi, Abreu, Thaís Guimarães Morato, Laus, José Luiz
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-05-2019; Springer Nature B.V
• Subjects: Blood vessels; Cornea; Eye (anatomy); Mathematical models; Medicine; Medicine & Public Health; Metronidazole; Ophthalmology; Original Paper; Rats; Rodents; Vascularization; Rats; Angiogenesis; Cornea; Metronidazole; Neovascularization
• ISSN: 0165-5701; 1573-2630
• DOI: 10.1007/s10792-018-0922-2

Purpose
To evaluate the effect of metronidazole ophthalmic solutions on corneal neovascularization (CNV) in a rat model.
Methods
A chemical burn was created in the right central cornea of 40 rats. Animals were randomized and distributed into four study groups (
n
 = 10 rats per group) designated Met_0.1%, Met_0.5%, sham, and untreated groups. Chemical-burned corneas in the Met_0.1% and Met_0.5% groups received ophthalmic solutions of 0.1 and 0.5% metronidazole, respectively. Corneas in the sham group received phosphate-buffered saline (metronidazole diluent). All treated eyes received ophthalmic solution at intervals of 6 h, for up to 30 days. Untreated corneas received no treatment. CNV was evaluated postinjury using corneal photographs at different evaluation time points. The main CNV outcome measures were: burn intensity, index of CNV, and percentage of vascularized corneal area. Five rats from each group were euthanized, on days 15 and 30; the samples were collected for histological analyses. Differences with
P
 < 0.05 were considered significant.
Results
CNV was observed in the eyes from day 7 postinjury. However, the indices of CNV for the Met_0.1% and Met_0.5% groups were smaller than those for the sham and untreated groups (
P
 < 0.05). Furthermore, corneas treated with 0.1 or 0.5% metronidazole had smaller vascularized areas compared to control corneas. On histological study, the presence of blood vessels confirmed clinical outcomes.
Conclusions
Regular instillation of 0.1 or 0.5% metronidazole had a significant inhibitory effect for CNV on chemical burns induced in a rat model.