Chlamydiaceae in riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without signs of reproductive disease

• Published in: New Zealand veterinary journal Vol. 60; no. 4; pp. 228 - 233
• Main Authors: Osman, KM, Ali, HA, ElJakee, JA, Galal, HM
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-07-2012
• Subjects: algorithms; Animals; antibodies; blood; buffaloes; Buffaloes - microbiology; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Cercopithecus aethiops; chicken eggs; Chlamydiaceae - isolation & purification; Chlamydiaceae Infections - epidemiology; Chlamydiaceae Infections - microbiology; Chlamydiaceae Infections - veterinary; Chlamydophila abortus; Chlamydophila infections; Chlamydophila psittaci; complement; Complement Fixation Tests; cow; Cows; disease detection; Diseases; Egypt - epidemiology; Female; genes; Genital Diseases, Female - epidemiology; Genital Diseases, Female - microbiology; Genital Diseases, Female - veterinary; polymerase chain reaction; Polymerase Chain Reaction - veterinary; Polymorphism, Restriction Fragment Length; Psittacosis; reproductive disorder; reproductive disorders; Reproductive health; restriction fragment length polymorphism; Retrospective Studies; Riverine buffalo; vaccines; Vero Cells; veterinary clinics; Water buffalo; Egypt
• ISSN: 1176-0710; 0048-0169; 1176-0710
• DOI: 10.1080/00480169.2012.668123

AIMS: To obtain information and compare the prevalence of Chlamydiaceae in riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without clinical signs of reproductive disease.METHODS: Vaginal swabs and blood samples were collected from animals attending Governmental Veterinary Clinics without (buffalo n=39, cows n=20) and with (buffalo n=63, cows n=53) signs of reproductive disease. Serum samples were tested for antibodies to Chlamydiaceae using complement fixation testing (CFT). Vaginal swabs were tested for Chlamydiaceae following inoculation into Vero cells and 6-day-old embryonated chicken eggs, using modified Giménez and immunoperoxidase staining, PCR analyses targeting the omp2 gene, and Restriction Fragment Length Polymorphism PCR (RFLP-PCR) for species identification.RESULTS: Antibodies to Chlamydiaceae were detected in 30/39 (77%) and 50/63 (79%) buffalo without and with signs of reproductive disease, respectively, and 10/20 (50%) and 39/53 (74%) of cows with and without signs of reproductive disease, respectively. Positive samples from PCR analysis were identified in 31/39 (79%) and 37/63 (59%) buffalo without and with signs of reproductive disease, respectively, and 12/20 (60%) and 46/53 (89%) of cows without and with signs of reproductive disease, respectively. Using RFLP-PCR, 57/68 (84%) of samples from buffalo, and 47/58 (81%) from cows, were identified as Chlamydophila psittaci and the reminder as Cp. abortus. From the CFT and PCR results there was no significant difference in the prevalence of positive samples between species, or between animals without or with signs of reproductive disease.CONCLUSION: The presence of anti-Chlamydiaceae antibodies in 77% of the animals with signs of reproductive disease and the detection of Chlamydiaceae in 72% of vaginal swabs of the animals suggest a pathogenic role by Chlamydiaceae in riverine buffalo and cows. The main Chlamydiaceae found in the genital tract of cattle in Egypt were Cp. psittaci and Cp. abortus.CLINICAL RELEVANCE: Chlamydophila spp. should be included in diagnostic algorithms for reproductive disorders, in order to assess the real burden of Chlamydophila associated disease in buffalo and cattle and to evaluate the potential value of vaccines.