Production of hydrogen by Enterobacter aerogenes in an immobilized cell reactor

• Published in: International journal of hydrogen energy Vol. 42; no. 14; pp. 9024 - 9030
• Main Authors: Satar, Ibdal, Ghasemi, Mostafa, Aljlil, Saad A., Isahak, Wan Nor Roslam Wan, Abdalla, Abdalla M., Alam, Javed, Daud, Wan Ramli Wan, Yarmo, Mohd Ambar, Akbarzadeh, Omid
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 06-04-2017
• Subjects: Carbon dioxide; Enterobacter aerogenes; Hydrogen; Immobilized cell reactor; Enterobacter aerogenes; Hydrogen; Immobilized cell reactor; Carbon dioxide
• ISSN: 0360-3199; 1879-3487
• DOI: 10.1016/j.ijhydene.2016.04.150

The production of hydrogen from glucose by using Enterobacter aerogenes ATCC 13048 (E. aerogenes) in an immobilized cell reactor (ICR) was investigated. The effect of several factors, such as the glucose concentration, feed flow rate, and fermentation time were examined. The highest amount of hydrogen (9.44 mmol H2/g glucose) was obtained at a glucose concentration of 8 g/L, flow rate of 0.5 mL/min, retention time of 24 h and at a temperature of 30 °C. Meanwhile, the highest amount of carbon dioxide (1.68 mmol CO2/g glucose) was obtained at a glucose concentration of 10 g/L, flow rate of 0.7 mL/min, hydraulic retention time of 24 h and at a temperature of 30 °C. The hydrogen and carbon dioxide production were affected by glucose concentration, hydraulic retention time (HRT) and fermentation time. This study showed that the ICR was a very efficient method for the production of hydrogen and carbon dioxide gases.
•Growing up and immobilization of Entercobacter aerogenes.•Production of hydrogen and carbon dioxide in an immobilized cell reactor.•Testing of different conditions for production of hydrogen.•Optimization of condition of production of hydrogen.