Mate tea (Ilex paraguariensis) improves bone formation in the alveolar socket healing after tooth extraction in rats

• Published in: Clinical oral investigations Vol. 22; no. 3; pp. 1449 - 1461
• Main Authors: Brasilino, Matheus da Silva, Stringhetta-Garcia, Camila Tami, Pereira, Camila Scacco, Pereira, Ariana Aparecida Ferreira, Stringhetta, Karina, Leopoldino, Andréia Machado, Crivelini, Marcelo Macedo, Ervolino, Edilson, Dornelles, Rita Cássia Menegati, de Melo Stevanato Nakamune, Ana Cláudia, Chaves-Neto, Antonio Hernandes
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2018; Springer Nature B.V
• Subjects: Acid phosphatase (tartrate-resistant); Alkaline phosphatase; Alkaline Phosphatase - blood; Alveolar bone; Animals; Antioxidants; Antioxidants - metabolism; Beverages; Bone density; Bone growth; Bone healing; Calcium; Calcium - blood; Dentistry; Ilex paraguariensis; Immunohistochemistry; Male; Malondialdehyde; Malondialdehyde - blood; Manganese; Medicine; Original Article; Osteocalcin; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - drug effects; Osteoprotegerin; Osteoprotegerin - metabolism; Phosphatase; Phosphorus; Phosphorus - blood; RANK Ligand - metabolism; Rats; Rats, Wistar; Rodents; Superoxide dismutase; Superoxide Dismutase - metabolism; Tartrate-Resistant Acid Phosphatase - metabolism; Tea; Teeth; Tooth Extraction; Tooth Socket - drug effects; TRANCE protein; Antioxidant; Tooth extraction; Wound healing; Ilex paraguariensis
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-017-2249-1

Objectives
The objective of this study was to investigate the effects of mate tea (MT) [
Ilex paraguariensis
] on alveolar socket healing after tooth extraction.
Materials and methods
Sixteen male rats were divided into MT and control groups. MT was administered by intragastric gavage at a dose of 20 mg/kg/day for 28 days before and 28 days after right maxillary incisor extraction. The control group received an equal volume of water. Histopathological and histometric analysis of the neoformed bone area and osteocyte density were performed, as well as immunohistochemical analysis of osteocalcin (OCN), receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase (TRAP), and manganese superoxide dismutase (MnSOD) in the alveolar socket. Calcium, phosphorus, alkaline phosphatase (ALP) activity, total antioxidant capacity (TAC), and malondialdehyde (MDA) were measured in plasma, whereas TRAP activity was determined in serum.
Results
Histometry evidenced an increase in bone area (
P
 < 0.0001) and osteocyte density (
P
 < 0.0001). MT increased immunolabeling of MnSOD (
P
 < 0.001), OCN (
P
 < 0.0001), RANKL (
P
 < 0.001), OPG (
P
 < 0.0001), and TRAP
(P
 < 0.001). Calcium and phosphorus concentrations did not differ between the groups. In addition, MT enhanced ALP (
P
 < 0.05) and TRAP (
P
 < 0.0001) activities. MT increased the TAC (
P
 < 0.001), whereas it reduced MDA concentrations (
P
 < 0.0001).
Conclusions
MT increases bone area and osteocyte density in the alveolar socket healing on day 28 after tooth extraction.
Clinical relevance
Regular MT ingestion improves the antioxidant defenses and bone formation, which is beneficial for alveolar socket bone healing after tooth extraction.