BMPR-II expression and OP-1 effects in developing chicken retinal explants

Low-Stringency PCR was used to isolate the chicken homologue of the human bone morphogenetic protein receptor type II (BMPR-II). In situ hybridization localized mRNA expression for this serine/threonine kinase receptor. It was weakly expressed in the vitreal side of the neural retina at E6. In the E...

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Bibliographic Details
Published in:	Neuroreport Vol. 9; no. 6; pp. 1097 - 1101
Main Authors:	Carri, Nestor G, Bengtsson, Henrik, Charette, Marc F, Ebendal, Ted
Format:	Journal Article
Language:	English
Published:	Hagerstown, MD Lippincott-Raven Publishers 20-04-1998 Lippincott Williams and Wilkins
Subjects:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Bone Morphogenetic Protein 7 Bone Morphogenetic Protein Receptors, Type II Bone Morphogenetic Proteins - pharmacology Cell Differentiation - drug effects Cell Survival - drug effects Chick Embryo Cloning, Molecular Development. Senescence. Regeneration. Transplantation Fundamental and applied biological sciences. Psychology Molecular Sequence Data Protein-Serine-Threonine Kinases - genetics Receptors, Cell Surface - genetics Retina - drug effects Retina - embryology Retinal Ganglion Cells - drug effects Transforming Growth Factor beta Vertebrates: nervous system and sense organs In situ Retina Hybridization Gene expression Eye Visual system Ganglion neuron Vertebrata Bone morphogenetic protein Messenger RNA Development Peptidergic receptor Chicken Aves
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Summary:	Low-Stringency PCR was used to isolate the chicken homologue of the human bone morphogenetic protein receptor type II (BMPR-II). In situ hybridization localized mRNA expression for this serine/threonine kinase receptor. It was weakly expressed in the vitreal side of the neural retina at E6. In the E7 chicken, fairly strong labelling was found in cells of the internal part of the neural retina. At E9, strong labelling was found in the region of the retinal ganglion cells. Explants of E6 retina exposed to osteogenic protein-1 (OP-1/BMP-7), exhibited dense retinal fibre outgrowth. This suggests that BMPR-II may form a signalling receptor complex important for retinal development. OP-1 and related ligands may serve functions supplementary to those of neurotrophins.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0959-4965 1473-558X
DOI:	10.1097/00001756-199804200-00026