Regulated expression of dual specificity protein phosphatases in rat brain

• Published in: Neuroreport Vol. 9; no. 18; pp. 4081 - 4086
• Main Authors: Boschert, Ursula, Dickinson, Robin, Muda, Marco, Camps, Montserrat, Arkinstall, Steve
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins, Inc 21-12-1998; Lippincott Williams and Wilkins
• Subjects: Aging - metabolism; Animals; Animals, Newborn - growth & development; Animals, Newborn - metabolism; Biological and medical sciences; Brain - enzymology; Cell Cycle Proteins; Development. Senescence. Regeneration. Transplantation; Dual Specificity Phosphatase 1; Dual Specificity Phosphatase 6; Dual-Specificity Phosphatases; Fundamental and applied biological sciences. Psychology; Immediate-Early Proteins - genetics; Immediate-Early Proteins - metabolism; In Situ Hybridization; Kainic Acid; Male; Phosphoprotein Phosphatases - metabolism; Protein Phosphatase 1; Protein Tyrosine Phosphatases - genetics; Protein Tyrosine Phosphatases - metabolism; Rats; Rats, Wistar; RNA, Messenger - metabolism; Seizures - chemically induced; Seizures - enzymology; Time Factors; Tissue Distribution - physiology; Vertebrates: nervous system and sense organs; Rat; Enzyme; Phosphoprotein phosphatase; Epilepsy; Rodentia; Central nervous system; Phosphoric monoester hydrolases; Esterases; Gene expression; Postnatal development; Vertebrata; Regulation(control); Mammalia; Hydrolases; Kainic acid; Brain (vertebrata)
• ISSN: 0959-4965; 1473-558X
• DOI: 10.1097/00001756-199812210-00014

ACTIVATED mitogen-activated protein (MAP) kinases play an essential role controlling many neuronal functions. Dual specificity protein phosphatases (DS-PTPs) elicit selective inactivation of MAP kinases and are under tight transcriptional control. We have studied expression of four DS-PTPs (MKP-1, MKP-X, MKP-3 and B23) in rat brain and examined changes during post-natal development and following kainic acid induced seizure activity. In normal adult brain these DS-PTPs exhibit a strikingly different expression pattern. Only MKP-1 was regulated during development with levels increased transiently (P15-P21) within the thalamus and somatosensory cortex. Following kainate treatment, MKP-1, MKP-3 and B23 all exhibit striking changes in expression within hippocampal subfields CA1-3 and dentate gyrus. Regulated transcription of DS-PTPs may play a critical role controlling MAP kinase dependent processes including synaptic remodeling and neuronal death.