Adipocyte Long Noncoding RNA Transcriptome Analysis of Obese Mice Identified Lnc-leptin Which Regulates Leptin

• Published in: Diabetes (New York, N.Y.) Vol. 67; no. 6; pp. 1045 - 1056
• Main Authors: Lo, Kinyui Alice, Huang, Shiqi, Esther Walet, Arcinas Camille, Zhang, Zhi-Chun, Khee-Shing Leow, Melvin, Liu, Meihui, Sun, Lei
• Format: Journal Article
• Language: English
• Published: United States American Diabetes Association 01-06-2018
• Subjects: Adipocytes; Adipogenesis; Adipose tissue; Deoxyribonucleic acid; DNA; Energy balance; Gene expression; Glucose; Hyperglycemia; Insulin; Leptin; Liver; Obesity; Ribonucleic acid; RNA
• ISSN: 0012-1797; 1939-327X
• DOI: 10.2337/db17-0526

Obesity induces profound transcriptome changes in adipocytes; recent evidence suggests that lncRNAs play key roles in this process. Here, we performed a comprehensive transcriptome study by RNA-Seq in adipocytes isolated from interscapular brown, inguinal and epididymal white adipose tissues in diet-induced obese mice. Our analysis reveals a set of obesity-dysregulated lncRNAs, many of which exhibit dynamic changes in fed
fasted state, potentially serving as novel molecular markers reflecting adipose energy status. Among the most prominent ones is
, an lncRNA transcribed from an enhancer region upstream of
Expression of
is sensitive to insulin and closely correlates to
expression across diverse pathophysiological conditions. Functionally, induction of
is essential for adipogenesis, and its presence is required for the maintenance of
expression
and
Direct interaction was detected between DNA loci of
and
in mature adipocytes, which diminished upon
knockdown. Our study establishes
as a new regulator of
.