Fluorescence determination of glyphosate based on a DNA-templated copper nanoparticle biosensor

• Published in: Mikrochimica acta (1966) Vol. 189; no. 4; p. 158
• Main Authors: Fang, Huajuan, Zhang, Xingping, Gao, Dongxu, Xiao, Yao, Ma, Liyuan, Yang, Hualin, Zhou, Yu
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-04-2022; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Biosensing Techniques; Biosensors; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Copper; Detectors; DNA; Fluorescence; Glycine - analogs & derivatives; Glyphosate; Metal Nanoparticles; Microengineering; Nanochemistry; Nanoparticles; Nanotechnology; Organophosphorus Compounds; Original Paper; Pesticides; Selectivity; Titration; Glyphosate; Rapid detection; DNA; Copper nanoparticles
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-022-05284-8

A rapid and convenient fluorescence glyphosate (GLYP) biosensor was developed based on DNA-templated copper nanoparticles (DNA-CuNPs). In the absence of GLYP, the DNA-CuNPs were formed through the reduction of Cu
2+
by vitamin C (Vc). The DNA-CuNPs emitted intense fluorescence at 615 nm when being excited at 340 nm. In the presence of GLYP, GLYP can strongly chelate with Cu
2+
by the phosphate and carboxyl groups to decrease the amount of free Cu
2+
. Due to the lack of free Cu
2+
, DNA-CuNPs cannot be formed, which caused the fluorescence to decrease. The whole detection process of this proposed GLYP biosensor can be completed within 14 min. Titration experiments showed that this biosensor had a linear relationship for GLYP in the range 1 to 18 µM with a limit of detection (LOD) of 0.47 µM. This biosensor showed obvious selectivity among other pesticides, even between GLYP and organophosphorus pesticides. This biosensor performed well for GLYP detection in real samples with recoveries of 88.0–104.0%.
Graphical abstract