Generation and characterization of human-derived iPSC lines from one pair of dizygotic twins discordant for schizophrenia

• Published in: Stem cell research Vol. 60; p. 102710
• Main Authors: Ni, Peiyan, Wang, Huiyao, Cai, Jia, Ran, Junzhe, Jiang, Youhui, Zhao, Liansheng, Wei, Jinxue, Ni, Rongjun, Wang, Yingcheng, Ma, Xiaohong, Wang, Qiang, Guo, Wanjun, Li, Tao
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01-04-2022; Elsevier
• Subjects: Cell Differentiation; Dizygotic twins; Female; Humans; Induced Pluripotent Stem Cells; Induced pluripotent stem cells (iPSCs); Kruppel-Like Factor 4; Leukocytes, Mononuclear; Male; Schizophrenia (SCZ); Schizophrenia - genetics; Twins, Dizygotic; Schizophrenia (SCZ); Dizygotic twins; Induced pluripotent stem cells (iPSCs)
• ISSN: 1873-5061; 1876-7753
• DOI: 10.1016/j.scr.2022.102710

Schizophrenia (SCZ) is a debilitating neurodevelopmental disorder with a high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were donated by a pair of dizygotic twins. The female was clinically diagnosed as SCZ by Diagnostic and Statistical Manual of Mental Disorders IV (DSM-IV) criteria, and her unaffected male sibling was healthy control. Induced pluripotent stem cells (iPSCs) were established using Episomal vectors carrying reprograming factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. These lines with normal karyotype highly expressed pluripotency markers and are capable to differentiate into derivatives of three germ layers. Both lines are negative of mycoplasma.