Head Transcriptomes of Two Closely Related Species of Fruit Flies of the Anastrepha fraterculus Group Reveals Divergent Genes in Species with Extensive Gene Flow

• Published in: G3 : genes - genomes - genetics Vol. 6; no. 10; pp. 3283 - 3295
• Main Authors: Rezende, Victor Borges, Congrains, Carlos, Lima, André Luís A, Campanini, Emeline Boni, Nakamura, Aline Minali, Oliveira, Janaína Lima de, Chahad-Ehlers, Samira, Junior, Iderval Sobrinho, Alves de Brito, Reinaldo
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01-10-2016; Oxford University Press
• Subjects: Alleles; Animals; Computational Biology - methods; de novo assembly; Evolution, Molecular; fixed SNPs; fraterculus group; Gene Expression Profiling; Gene Flow; Genes, Insect; Genetic Variation; High-Throughput Nucleotide Sequencing; Investigations; Molecular Sequence Annotation; next generation sequencing; Organ Specificity - genetics; Polymorphism, Single Nucleotide; positive selection; RNA-Seq; Selection, Genetic; Species Specificity; Tephritidae - genetics; Transcriptome; positive selection; de novo assembly; RNA-Seq; next generation sequencing; fraterculus group; fixed SNPs
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1534/g3.116.030486

Several fruit flies species of the Anastrepha fraterculus group are of great economic importance for the damage they cause to a variety of fleshy fruits. Some species in this group have diverged recently, with evidence of introgression, showing similar morphological attributes that render their identification difficult, reinforcing the relevance of identifying new molecular markers that may differentiate species. We investigated genes expressed in head tissues from two closely related species: A. obliqua and A. fraterculus, aiming to identify fixed single nucleotide polymorphisms (SNPs) and highly differentiated transcripts, which, considering that these species still experience some level of gene flow, could indicate potential candidate genes involved in their differentiation process. We generated multiple libraries from head tissues of these two species, at different reproductive stages, for both sexes. Our analyses indicate that the de novo transcriptome assemblies are fairly complete. We also produced a hybrid assembly to map each species' reads, and identified 67,470 SNPs in A. fraterculus, 39,252 in A. obliqua, and 6386 that were common to both species. We identified 164 highly differentiated unigenes that had a mean interspecific index ([Formula: see text]) of at least 0.94. We selected unigenes that had Ka/Ks higher than 0.5, or had at least three or more highly differentiated SNPs as potential candidate genes for species differentiation. Among these candidates, we identified proteases, regulators of redox homeostasis, and an odorant-binding protein (Obp99c), among other genes. The head transcriptomes described here enabled the identification of thousands of genes hitherto unavailable for these species, and generated a set of candidate genes that are potentially important to genetically identify species and understand the speciation process in the presence of gene flow of A. obliqua and A. fraterculus.