Prehabilitation Ameliorates Gut Ischemia Reperfusion Injury in Mice

• Published in: The Journal of surgical research Vol. 282; pp. 71 - 83
• Main Authors: Takahashi, Kazuya, Higashizono, Kazuya, Fukatsu, Kazuhiko, Murakoshi, Satoshi, Takayama, Haruka, Noguchi, Midori, Matsumoto, Nana, Seto, Yasuyuki
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2023
• Subjects: 8-Hydroxy-2'-Deoxyguanosine; 8-OHdG; Adenosine Triphosphate; Animals; Antioxidants; Cytokine; Cytokines; Gut ischemia reperfusion; Ischemia; Male; Mice; Mice, Inbred C57BL; MPO; Peroxidase; Physical Conditioning, Animal; Prehabilitation; Preoperative Exercise; Reperfusion Injury - prevention & control; Gut ischemia reperfusion; Prehabilitation; MPO; Adenosine triphosphate; Cytokine; 8-OHdG
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2022.08.033

We previously demonstrated that prehabilitation by running on a treadmill leads to improved survival after gut ischemia reperfusion (I/R) in mice. The purpose of this research was to examine whether prehabilitation attenuates inflammatory responses after gut I/R in mice.
Male C57BL/6J mice (n = 92) were assigned to the sedentary (n = 46) or the exercise (n = 46) group. The exercise group ran on a treadmill for 4 wk, while the sedentary mice did not exercise. After the 4-week pretreatment, all mice underwent gut I/R and the blood, urine, small intestine, lung, liver, and gastrocnemius were harvested prior to ischemia or at 0, 3, 6, or 24 h after reperfusion. Histologically demonstrated organ damage, cytokine levels in the blood, gut and gastrocnemius, myeloperoxidase activity in the gut, 8-hydroxy-2′-deoxyguanosine levels in urine and the gut, and adenosine triphosphate (ATP) and ATP + ADP + adenosine monophosphate levels in the gut and gastrocnemius were evaluated.
The treadmill exercise reduced gut and lung injuries at 3 h and liver injury at 6 h after reperfusion. Running on the treadmill also decreased proinflammatory cytokine levels in the blood at 6 h, gut at 3 h and gastrocnemius at 6 h after reperfusion, myeloperoxidase activity in the gut prior to ischemia, and 6 h after reperfusion and the urinary 8-hydroxy-2′-deoxyguanosine level at 24 h after reperfusion, while ATP levels in exercised mice prior to ischemia and 3 h after reperfusion were increased in the intestine as compared to the levels in sedentary mice.
Prehabilitation with treadmill exercise reduces inflammatory responses after gut I/R and may exert protective actions against gut I/R.