A contribution to the systematization of bovine herpesvirus 1 based on genomic mapping by restriction fragment pattern analysis

• Published in: Virus research Vol. 46; no. 1; pp. 177 - 182
• Main Authors: Christensen, L.S., Madsen, K.G., Nylin, B., Rønsholt, L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-1996
• Subjects: Animals; Bacterial Proteins; bovin; bovine herpesvirus; Bovine herpesvirus 1; carte genetique; Cattle; Deoxyribonuclease HindIII; Deoxyribonucleases, Type II Site-Specific; Electrophoresis, Agar Gel; ganado bovino; genetic maps; genomas; genome; Genome, Viral; genomes; Herpesvirus 1, Bovine - classification; Herpesvirus 1, Bovine - genetics; Herpesvirus 1, Bovine - isolation & purification; herpesvirus bovin; herpesvirus bovino; mapas geneticos; Restriction fragment pattern analysis; Restriction Mapping - methods; rflp; vaccin; Vaccine; vaccines; vacuna; Restriction fragment pattern analysis; Bovine herpesvirus 1; Vaccine
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/S0168-1702(96)01383-4

Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infections bovine rhinotracheitis and the variants originally associated with infectious pustular vulvovaginitis were identified. These findings, as well as the lack of any correlation between genome type and clinical manifestation, confirm that there is no phylogenetic basis for a distinction between groups of strains associated with genital and respiratory disease. Two attenuated vaccine strains can be identified as deviating from field isolates.