Quantitative analysis of essential oils in perfume using multivariate curve resolution combined with comprehensive two-dimensional gas chromatography

• Published in: Analytica chimica acta Vol. 699; no. 1; pp. 120 - 125
• Main Authors: de Godoy, Luiz Antonio Fonseca, Hantao, Leandro Wang, Pedroso, Marcio Pozzobon, Poppi, Ronei Jesus, Augusto, Fabio
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05-08-2011; Elsevier
• Subjects: Algorithms; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Gas - instrumentation; Chromatography, Gas - methods; Comprehensive two-dimensional gas chromatography; Cymbopogon - chemistry; Exact sciences and technology; Flame Ionization; Gas chromatographic methods; Least-Squares Analysis; Lemon grass essential oil; Multivariate analysis; Multivariate curve resolution; Oils, Volatile - analysis; Oils, Volatile - isolation & purification; Perfume; Perfume - chemistry; Rosemary essential oil; Rosmarinus; Rosmarinus - chemistry; Multivariate analysis; Comprehensive two-dimensional gas chromatography; Multivariate curve resolution; Lemon grass essential oil; Perfume; Rosemary essential oil; Second order; Two dimensional chromatography; Alcohol; Flame ionization detector; Optimization; Mean square error; Target; Accuracy; Instrumental response; Standard curve; Essential oil; Solvent; Quantitative analysis; Root; Sample; Prediction; Flame ionization detection; Calibration; Method; Algorithm; Gas chromatography; Cereal
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2011.05.003

[Display omitted]
► Multivariate curve resolution (MCR) was assessed as a quantitative tool for GC
×
GC. ► Quantitation of essential oils on perfume was made using GC
×
GC-FID chromatograms. ► The combination GC
×
GC
+
MCR resulted on excellent accuracy and precision. ► It can be an alternative to quantify ingredients on complex mixtures.
The use of multivariate curve resolution (MCR) to build multivariate quantitative models using data obtained from comprehensive two-dimensional gas chromatography with flame ionization detection (GC
×
GC-FID) is presented and evaluated. The MCR algorithm presents some important features, such as second order advantage and the recovery of the instrumental response for each pure component after optimization by an alternating least squares (ALS) procedure. A model to quantify the essential oil of rosemary was built using a calibration set containing only known concentrations of the essential oil and cereal alcohol as solvent. A calibration curve correlating the concentration of the essential oil of rosemary and the instrumental response obtained from the MCR-ALS algorithm was obtained, and this calibration model was applied to predict the concentration of the oil in complex samples (mixtures of the essential oil, pineapple essence and commercial perfume). The values of the root mean square error of prediction (RMSEP) and of the root mean square error of the percentage deviation (RMSPD) obtained were 0.4% (v/v) and 7.2%, respectively. Additionally, a second model was built and used to evaluate the accuracy of the method. A model to quantify the essential oil of lemon grass was built and its concentration was predicted in the validation set and real perfume samples. The RMSEP and RMSPD obtained were 0.5% (v/v) and 6.9%, respectively, and the concentration of the essential oil of lemon grass in perfume agreed to the value informed by the manufacturer. The result indicates that the MCR algorithm is adequate to resolve the target chromatogram from the complex sample and to build multivariate models of GC
×
GC-FID data.