Immunomagnetic T-lymphocyte depletion (ITLD) of rat bone marrow using OX-19 monoclonal antibody

Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (OX19) bound to monosized, magnetic, polymer beads, T lymphocytes were...

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Bibliographic Details
Published in:	Journal of investigative surgery Vol. 2; no. 3; p. 241
Main Authors:	Hoffman, A L, Makowka, L, Cramer, D V, Cai, X, Pascualone, A, Banner, B F, Sheahan, D G, Rettinger, P, Starzl, T E
Format:	Journal Article
Language:	English
Published:	United States 1989
Subjects:	Animals Antibodies, Monoclonal - immunology Bone Marrow Cells Bone Marrow Transplantation - immunology Cell Separation - instrumentation Cell Separation - methods Flow Cytometry Graft vs Host Disease - prevention & control Lymphocyte Depletion Magnetics Rats Rats, Inbred Lew - immunology Rats, Inbred Strains - immunology T-Lymphocytes - immunology
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Summary:	Graft versus host disease (GVHD) may be abrogated and host survival prolonged by in vitro depletion of T lymphocytes from bone marrow (BM) prior to allotransplantation. Using a mouse anti-rat pan T-lymphocyte monoclonal antibody (OX19) bound to monosized, magnetic, polymer beads, T lymphocytes were removed in vitro from normal bone marrow. The removal of the T lymphocytes was confirmed by flow cytometry. Injection of the T-lymphocyte-depleted bone marrow into fully allogeneic rats prevents the induction of GVHD and prolongs host survival.
ISSN:	0894-1939
DOI:	10.3109/08941938909057430