Babesial antibody dynamics after cattle immunisation with live vaccines, measured with an indirect immunofluorescence test

• Published in: Veterinary parasitology Vol. 70; no. 1; pp. 33 - 39
• Main Authors: Guglielmone, A.A., Lugaresi, C.I., Volpogni, M.M., Anziani, O.S., Vanzini, V.R.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-1997
• Subjects: Anaplasma - immunology; Animals; Antibodies, Protozoan - blood; Antibody dynamics; Babesia - immunology; BABESIA BIGEMINA; BABESIA BOVIS; Bacterial Vaccines - immunology; BOVIN; CATTLE; Cattle - immunology; Fluorescent Antibody Technique, Indirect - veterinary; GANADO BOVINO; IFA test; IMMUNE RESPONSE; Immunisation-Protozoa; IMMUNOFLUORESCENCE; INMUNOFLUORESCENCIA; LIVE VACCINES; Male; Protozoan Vaccines - immunology; REPONSE IMMUNITAIRE; RESPUESTA INMUNOLOGICA; VACCIN VIVANT; Vaccination - veterinary; Vaccines; Vaccines, Attenuated - immunology; Vaccines, Combined - immunology; VACUNA VIVA; Argentina; IFA test; Babesia bigemina; Immunisation-Protozoa; Antibody dynamics; Babesia bovis; Vaccines
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/S0304-4017(96)01143-0

The efficacy of vaccination of Argentinean cattle against babesiosis and anaplasmosis using live immunogens was tested to detect specific antibodies in samples obtained about 60 days after vaccination. Under these conditions a higher than expected proportion of cattle failed to show antibodies against
Babesia bigemina. Therefore, a study was designed to evaluate if this failure was due to insensitivity of the routine test to detect antibodies to
B. bigemina or to lack of infectivity of the live vaccine.
Four groups (G) of cattle were each inoculated subcutaneously with 10 million
Babesia bovis (vaccinal strain R1A), 10 million
B. bigemina (vaccinal strain S1A) and 10 million
Anaplasma centrale (strain M1). G1 and G2 consisted of ten Angus bulls 20–24 months old and ten Angus bulls 15–18 months old, respectively; G3 and G4 consisted of ten and 16 Holstein 1-month-old male calves, respectively. Blood samples were obtained on days 0, 10, 20, 30, 40, 50 and 60 after vaccination and the sera were analysed with an indirect immunofluorescent (IFA) test to detect antibodies to
B. bovis (baseline dilution for a positive result 1:60) and
B. bigemina (baseline dilution 1:120). Positive IFA titres were considered as evidence of the infectivity of the
Babesia vaccinal strains contained in the vaccine.
All Angus bulls were found positive to antibodies against both
Babesia species, by day 20 (
B. bovis) and day 30 (
B. bigemina), whereas 10–25% of Holstein calves were negative throughout. The partial lack of vaccine infectivity in the calves was considered to be a consequence of innate resistance of young calves to
Babesia. Antibody titres to
B. bovis and
B. bigemina declined by day 60 after vaccination. However, all cattle that were positive to
B. bovis antibodies on day 50 were still positive to the IFA test 10 days later while 10%, 30% and 12% of cattle of G1, G2 and G3 that were positive to
B. bigemina antibodies on day 50 after vaccination were found negative to the IFA test on day 60. In future, samples taken on days 40–50 will be used for detection of
B. bigemina antibodies in vaccinated cattle, on day 60 for
A. centrale and on either occasion for
B. bovis.
The reaction to the inoculation of
B. bigemina S1A strain appears to lag behind the reaction to
B. bovis R1A strain. It is not certain if this is a normal reaction to this
B. bigemina strain or the result of interaction with the
B. bovis strain.