The NKR-P1B Gene Product Is an Inhibitory Receptor on SJL/J NK Cells

• Published in: The Journal of immunology (1950) Vol. 162; no. 10; pp. 5876 - 5887
• Main Authors: Kung, Sam K. P, Su, Ruey-Chyi, Shannon, John, Miller, Richard G
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15-05-1999
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antibody Specificity; Antigens; Antigens, Surface - isolation & purification; Clone Cells; Cytotoxicity, Immunologic; Hybridomas; Intracellular Signaling Peptides and Proteins; Killer Cells, Natural - immunology; Lectins, C-Type; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred DBA; Molecular Sequence Data; NK Cell Lectin-Like Receptor Subfamily B; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatase, Non-Receptor Type 6; Protein Tyrosine Phosphatases; Proteins; Receptors, Immunologic - isolation & purification; Sequence Analysis; Sequence Homology, Amino Acid; Species Specificity
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.162.10.5876

The mouse NKR-P1 family includes at least three genes: NKR-P1A, -B, -C. Neither surface expression nor function of the NKR-P1B gene product has previously been shown. Here, we demonstrate that the SJL/J allele of the NKR-P1B gene product is expressed on SJL/J NK cells, and is recognized by PK136 mAb. Interestingly, the same mAb does not recognize the NKR-P1B gene product of C57BL/6. We have also generated a novel mAb, 1C10, that recognizes an activation receptor on SJL/J NK cells. Activation of the NKR-P1B receptor-inhibited 1C10 mAb induced redirected lysis and recruited SHP-1, indicating that NKR-P1B is an inhibitory receptor. Therefore, the mouse NKR-P1 gene family, like the Ly49 family, includes both activation and inhibitory receptors.