A promising technology for wound healing; in-vitro and in-vivo evaluation of chitosan nano-biocomposite films containing gentamicin

• Published in: Journal of microencapsulation Vol. 38; no. 2; pp. 100 - 107
• Main Authors: Asgarirad, Hossein, Ebrahimnejad, Pedram, Mahjoub, Mohammad Ali, Jalalian, Mohammad, Morad, Hamed, Ataee, Ramin, Hosseini, Seyyedeh Saba, Farmoudeh, Ali
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 17-02-2021
• Subjects: Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - therapeutic use; Biocomposite; chitosan; Chitosan - chemistry; Drug Carriers - chemistry; Drug Liberation; gentamicin; Gentamicins - administration & dosage; Gentamicins - chemistry; Gentamicins - therapeutic use; ionic gelation; Nanoparticles - chemistry; Rats; Rats, Wistar; Skin - drug effects; Skin - injuries; Skin - pathology; Wound Healing - drug effects; wound-healing; ionic gelation; gentamicin; chitosan; Biocomposite; wound-healing
• ISSN: 0265-2048; 1464-5246
• DOI: 10.1080/02652048.2020.1851789

Aim: This paper aims to study in-vitro and in-vivo evaluation of chitosan (CHI) biocomposite of gentamicin nanoparticles (GNPs) for wound healing.
Methods: In this study, CHI nanoparticles (NPs) were prepared using the ionic gelation technique. GNP biocomposites were examined on the excision wound model in Wistar rats to determine the in-vivo efficiency.
Results: The diameter and zeta potential of NPs were between 151-212.9 nm and 37.2 − 51.1 mV, respectively. The entrapment efficiency was in an acceptable range of 36.6-42.7% w/w. The release test information was fitted to mathematical models (Zero, First order, Higuchi, and Korsmeyer-Peppas), and according to calculations, the kinetics of drug release followed the Korsmeyer-Peppas model. A comparison of thermograms revealed that the drug was present in the formulation in a non-crystalline form.
Conclusion: Histological studies of the wound showed that the rate of skin tissue repair was higher in the GNP biocomposite treatment group than in the others.