Optical demodulation system for digitally encoded suspension array in fluoroimmunoassay

• Published in: Journal of biomedical optics Vol. 22; no. 9; p. 097003
• Main Authors: He, Qinghua, Li, Dongmei, He, Yonghong, Guan, Tian, Zhang, Yilong, Shen, Zhiyuan, Chen, Xuejing, Liu, Siyu, Lu, Bangrong, Ji, Yanhong
• Format: Journal Article
• Language: English
• Published: United States Society of Photo-Optical Instrumentation Engineers 01-09-2017
• Subjects: Antibodies, Anti-Idiotypic - analysis; Fluoroimmunoassay - methods; Lasers; Sensitivity and Specificity; Spectrometry, Fluorescence - methods; laser-induced breakdown spectroscopy; suspension array; optical demodulation
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.JBO.22.9.097003

A laser-induced breakdown spectroscopy and fluorescence spectroscopy-coupled optical system is reported to demodulate digitally encoded suspension array in fluoroimmunoassay. It takes advantage of the plasma emissions of assembled elemental materials to digitally decode the suspension array, providing a more stable and accurate recognition to target biomolecules. By separating the decoding procedure of suspension array and adsorption quantity calculation of biomolecules into two independent channels, the cross talk between decoding and label signals in traditional methods had been successfully avoided, which promoted the accuracy of both processes and realized more sensitive quantitative detection of target biomolecules. We carried a multiplexed detection of several types of anti-IgG to verify the quantitative analysis performance of the system. A limit of detection of 1.48×10−10  M was achieved, demonstrating the detection sensitivity of the optical demodulation system.