Biochemical characterization and enhanced production of endoxylanase from thermophilic mould Myceliophthora thermophila

• Published in: Bioprocess and biosystems engineering Vol. 44; no. 7; pp. 1539 - 1555
• Main Authors: Dahiya, Seema, Kumar, Anil, Malik, Vinay, Kumar, Vinod, Singh, Bijender
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-07-2021; Springer Nature B.V
• Subjects: 6-Phytase - chemistry; Ammonium; Ammonium sulfate; Animal Feed; Biotechnology; Chemistry; Chemistry and Materials Science; Chromatography, Gel; Endo-1,4-beta Xylanases - chemistry; Environmental Engineering/Biotechnology; Enzymatic activity; Enzyme activity; Enzymes; Fermentation; Food Science; Gel electrophoresis; Gel filtration; Hexanes; Homogeneity; Hydrogen-Ion Concentration; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; n-Hexane; Octoxynol - chemistry; Optimization; Organic Chemicals; Organic solvents; Oryza; Phytase; Poultry; Poultry feed; Proteins; Research Paper; Rice straw; Sodium lauryl sulfate; Solid state fermentation; Solvents - chemistry; Sordariales - metabolism; Sugar; Sugars - chemistry; Surface-Active Agents - chemistry; Synergistic effect; Temperature; Water - chemistry; Endoxylanase; Poultry feed; Purification; Nutrition; Production; Myceliophthora thermophila
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-021-02539-1

Endoxylanase production from
M. thermophila
BJTLRMDU3 using rice straw was enhanced to 2.53-fold after optimization in solid state fermentation (SSF). Endoxylanase was purified to homogeneity employing ammonium sulfate precipitation followed by gel filtration chromatography and had a molecular mass of ~ 25 kDa estimated by SDS-PAGE. Optimal endoxylanase activity was recorded at pH 5.0 and 60 °C. Purified enzyme showed complete tolerance to
n
-hexane, but activity was slightly inhibited by other organic solvents. Among surfactants, Tweens (20, 60, and 80) and Triton X 100 slightly enhanced the enzyme activity. The
V
max
and
K
m
values for purified endoxylanase were 6.29 µmol/min/mg protein and 5.4 mg/ml, respectively. Endoxylanase released 79.08 and 42.95% higher reducing sugars and soluble proteins, respectively, which control after 48 h at 60 °C from poultry feed. Synergistic effect of endoxylanase (100 U/g) and phytase (15 U/g) on poultry feed released higher amount of reducing sugars (58.58 mg/feed), soluble proteins (42.48 mg/g feed), and inorganic phosphate (28.34 mg/feed) in contrast to control having 23.55, 16.98, and 10.46 mg/feed of reducing sugars, soluble proteins, and inorganic phosphate, respectively, at 60 °C supplemented with endoxylanase only.