Transformation of floral organs with GFP in Medicago truncatula

• Published in: Plant cell reports Vol. 19; no. 7; pp. 647 - 653
• Main Authors: KAMATE, K, RODRIGUEZ-LLORENTE, I. D, SCHOLTE, M, DURAND, P, RATET, P, KONDOROSI, E, KONDOROSI, A, TRINH, T. H
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-06-2000; Springer Nature B.V; Springer Verlag
• Subjects: Alfalfa; Biological and medical sciences; Biotechnology; Embryonic growth stage; Flowers; Fundamental and applied biological sciences. Psychology; Genes; Genetic engineering; Genetic technics; Life Sciences; Medicago truncatula; Methods. Procedures. Technologies; Proteins; Transgenic animals and transgenic plants; Transgenic plants; Vegetal Biology; Embryonic development; Flower; Vegetals; Genetic transformation; Agrobacterium tumefaciens; Tissue culture; Explant; Gene expression; Regeneration; Reporter gene; Leguminosae; Dicotyledones; Angiospermae; Green fluorescent protein; Bacteria; Spermatophyta; Transgenic plant; Rhizobiaceae; gfp; Medicago truncatula; Key words Floral organs
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002999900168

A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this method allows us to save time and to use a smaller greenhouse surface for the culture of donor plants. Southern hybridization showed that the internal gfp fragment had the expected size and the number of T-DNA copies integrated in the plant genome varied between one and three. These data suggest that the presence of the GFP protein has no toxic effects, since no rearrangement of the gfp reporter gene was detected in the regenerated plants.