Cellulolytic enzyme production from agricultural residues for biofuel purpose on circular economy approach

• Published in: Bioprocess and biosystems engineering Vol. 42; no. 5; pp. 677 - 685
• Main Authors: Astolfi, Viviane, Astolfi, Angela Luiza, Mazutti, Marcio A., Rigo, Elisandra, Di Luccio, Marco, Camargo, Aline Frumi, Dalastra, Caroline, Kubeneck, Simone, Fongaro, Gislaine, Treichel, Helen
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-05-2019; Springer Nature B.V
• Subjects: Agronomy; Biofuels; Biotechnology; Carboxymethyl cellulose; Carboxymethylcellulose; Cellulase; Cellulase - chemistry; Cellulase - isolation & purification; Cellulolytic enzymes; Cellulose; Chemistry; Chemistry and Materials Science; Circular economy; Crop residues; Endoglucanase; Environmental Engineering/Biotechnology; Enzymatic activity; Enzymes; Food Science; Freezing; Fungal Proteins - chemistry; Fungal Proteins - isolation & purification; Glycine max - chemistry; Hot Temperature; Hulls; Hydrogen-Ion Concentration; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Low temperature; Research Paper; Residues; Saccharification; Soybeans; Substrates; Sugar; Trichoderma - enzymology; Xylan; Xylanase; Saccharification; Solid-state fermentation; Agricultural residues; NRRL 3652; Trichoderma reesei NRRL 3652
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-019-02072-2

This study evaluated the production of cellulolytic enzymes from different agricultural residues. The crude enzyme extract produced was characterized and applied for saccharification of some agricultural residues. Maximum cellulolytic activities were obtained using soybean hulls. All enzymatic activities were highly stable at 40 °C at a pH range of 4.5–5.5. For stability at low temperatures, the enzyme extract was stored at freezing temperature and cooling for about 290 days without major loss of activity. The
K
m
values found for total cellulase (FPase), endoglucanase (CMCase), and xylanase were 19.73 mg ml
−1
, 0.65 mg ml
−1
, and 22.64 mg ml
−1
, respectively, and
V
max
values were 0.82 mol min
−1
 mg
−1
, 0.62 mol min
−1
 mg
−1
, and 104.17 mol min
−1
 mg
−1
to cellulose, carboxymethyl cellulose, and xylan, respectively. In the saccharification tests, the total amount of total reducing sugars (TRS) released from 1 g of soybean hulls catalyzed by the enzymes present in the crude enzyme extract was 0.16 g g
−1
dry substrate.