Epoxyeicosatrienoic acid prevents postischemic electrocardiogram abnormalities in an isolated heart model

• Published in: Journal of molecular and cellular cardiology Vol. 46; no. 1; pp. 67 - 74
• Main Authors: Batchu, S.N, Law, E, Brocks, D.R, Falck, J.R, Seubert, J.M
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-01-2009
• Subjects: Adenosine Triphosphate - chemistry; Animals; Cardiovascular; Cytochrome P-450 Enzyme System - metabolism; Eicosanoids - metabolism; Electrocardiography - methods; Enzyme Inhibitors - pharmacology; Epoxyeicosatrienoic acid; Heart - physiology; Humans; Ischemia; Ischemia–reperfusion; K + channels; Mice; Mice, Inbred C57BL; Mice, Transgenic; Myocardium - metabolism; Potassium - chemistry; QTc interval; Reperfusion Injury; ST elevation (STE); QTc interval; K + channels; Epoxyeicosatrienoic acid; Ischemia–reperfusion; ST elevation (STE)
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1016/j.yjmcc.2008.09.711

Abstract Cytochrome P450 epoxygenases metabolize arachidonic acid (AA) to epoxyeicosatrienoic acids (EETs) which are in turn converted to dihydroxyeicosatrienoic acids (DHETs) by soluble epoxide hydrolase (sEH). The main objective of this study was to investigate the protective effects of EETs following ischemic injury using an ex vivo electrocardiogram (EKG) model. Hearts from C57Bl/6, transgenic mice with cardiomyocyte-specific overexpression of CYP2J2 (Tr) and wildtype (WT) littermates were excised and perfused with constant pressure in a Langendorff apparatus. Electrodes were placed superficially at the right atrium and left ventricle to assess EKG waveforms. In ischemic reperfusion experiments hearts were subjected to 20 min of global no-flow ischemia followed by 20 min of reperfusion (R20). The EKG from C57Bl/6 hearts perfused with 1 μM 14,15-EET showed less QT prolongation (QTc) and ST elevation (STE) (QTc = 41 ± 3, STE = 2.3 ± 0.3; R20: QTc = 42 ± 2 ms, STE = 1.2 ± 0.2mv) than control hearts (QTc = 36 ± 2, STE = 2.3 ± 0.2; R20: QTc = 53 ± 3 ms; STE = 3.6 ± 0.4mv). Similar results of reduced QT prolongation and ST elevation were observed in EKG recording from CYP2J2 Tr mice (QTc = 35 ± 1, STE = 1.9 ± 0.1; R20: QTc = 38 ± 4 ms, STE = 1.3 ± 0.2mv) compared to WT hearts. The putative epoxygenase inhibitor MS-PPOH (50 μM) and EET antagonist 14,15-EEZE (10 μM) both abolished the cardioprotective response, implicating EETs in this process. In addition, separate exposure to the KATP channel blockers glibenclamide (1 μM) and HMR1098 (10 μM), or the PKA protein inhibitor H89 (50 nM) during reperfusion abolished the improved repolarization in both the models. Consistent with a role of PKA, CYP2J2 Tr mice had an enhanced activation of the PKAα regulatory II subunit in plasma membrane following IR injury. The present data demonstrate that EETs can enhance the recovery of ventricular repolarization following ischemia, potentially by facilitating activation of K+ channels and PKA-dependent signaling.