The Anti-Tumorigenic Activity of Sema3C in the Chick Embryo Chorioallantoic Membrane Model

• Published in: International journal of molecular sciences Vol. 20; no. 22; p. 5672
• Main Authors: Valiulytė, Indrė, Curkūnavičiūtė, Rūta, Ribokaitė, Laura, Kazlauskas, Arunas, Vaitkevičiūtė, Monika, Skauminas, Kęstutis, Valančiūtė, Angelija
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12-11-2019; MDPI
• Subjects: Angiogenesis; Animals; Antiangiogenics; Brain cancer; Cancer therapies; Cell activation; Cell growth; Cell Line, Tumor; Chick Embryo; Chorioallantoic membrane; Chorioallantoic Membrane - metabolism; Chorioallantoic Membrane - pathology; Embryos; Gastric cancer; Glioblastoma - drug therapy; Glioblastoma - genetics; Glioblastoma - metabolism; Glioblastoma - pathology; Glioma cells; Humans; Medical prognosis; Neoplasm Invasiveness; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - genetics; Neoplasms, Experimental - metabolism; Neoplasms, Experimental - pathology; Neovascularization, Pathologic - drug therapy; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - metabolism; Neovascularization, Pathologic - pathology; Nuclei (cytology); Propidium iodide; Proteins; Rac1 protein; Semaphorins - genetics; Semaphorins - metabolism; Signal processing; Stem cells; Survival; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Tumors; Valproic Acid - pharmacology; invasiveness; Sema3C; glioblastoma; cell proliferation; angiogenesis; cell adhesion; chicken embryo chorioallantoic membrane model; sodium valproate
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20225672

Sema3C protein, a member of the class 3 family of secreted semaphorins, play an important role in tumor development by regulating cell proliferation, migration, invasion, and angiogenesis processes. Depending on the type and malignancy grade of the tumor, Sema3C function remains controversial. In this study, we constructed a stably overexpressing Sema3C glioblastoma cell line U87 MG and tested it on the chicken embryo chorioallantoic membrane (CAM) model with the aim to reveal Sema3C protein function on angiogenesis process in ovo. Our experiments showed that Sema3C not only affects angiogenesis of CAM by inhibiting neovascularization but also acts as an anti-tumorigenic molecule by hampering U87 MG cell invasion into mesenchyme. The effects of Sema3C on CAM were similar to the effects of anti-epileptic drug sodium valproate (NaVP). Both, anti-angiogenic and anti-tumorigenic activities of Sema3C were enhanced by the treatment of NaVP and, importantly, were not attributed to the cytotoxic effects. Our studies suggest that Sema3C could be a promising target for glioblastoma treatment.