Microbial and metabolic features in renal transplant recipients with post‐transplantation diabetes mellitus

• Published in: International journal of urology Vol. 30; no. 6; pp. 504 - 513
• Main Authors: He, Jiayang, Yang, Mengchang, Quan, Xiangming, Wen, Jingyu, Lan, Yang, Yang, Hongji, Zhao, Gaoping, Hou, Yifu, Lu, Jun, Xu, Linfeng, Wei, Liang
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01-06-2023
• Subjects: Butyric acid; Correlation analysis; Diabetes; Diabetes mellitus; Diabetes Mellitus - etiology; Feces; gut microbiome; Humans; Intestinal microflora; Kidney Transplantation - adverse effects; Kidney transplants; Metabolic disorders; Metabolism; Metabolites; metabolome; Metabolomics; Microbiomes; post‐transplantation diabetes mellitus; renal transplantation; Risk Factors; Transplant Recipients; Transplantation; Tryptophan; post-transplantation diabetes mellitus; gut microbiome; renal transplantation; metabolome
• ISSN: 0919-8172; 1442-2042
• DOI: 10.1111/iju.15158

Objective
Post‐transplantation diabetes mellitus (PTDM) is a common complication in renal transplant recipients (RTRs). Gut microbiome plays important roles in a variety of chronic metabolic diseases, but its association with the occurrence and development of PTDM is still unknown. The present study integrates the analysis of gut microbiome and metabolites to further identify the characteristics of PTDM.
Methods
A total of 100 RTRs fecal samples were collected in our study. Among them, 55 samples were submitted to Hiseq sequencing, and 100 samples were used for non‐targeted metabolomics analysis. The gut microbiome and metabolomics of RTRs were comprehensively characterized.
Results
The species Dialister invisus was significantly associated with fasting plasma glucose (FPG). The functions of tryptophan and phenylalanine biosynthesis were enhanced in RTRs with PTDM, while the functions of fructose and butyric acid metabolism were reduced. Fecal metabolome analysis indicated that RTRs with PTDM had unique metabolite distribution characteristics, and two differentially expressed specific metabolites were significantly correlated with FPG. The correlation analysis of gut microbiome and metabolites showed that gut microbiome had an obvious effect on the metabolic characteristics of RTRs with PTDM. Moreover, the relative abundance of microbial function is associated with the expression of several specific gut microbiome and metabolites.
Conclusions
Our study identified the characteristics of gut microbiome and fecal metabolites in RTRs with PTDM, and we also found two important metabolites and a bacterium were significantly associated with PTDM, which might be used as novel targets in the research field of PTDM.