Structure of the Aspergillus niger pelA gene and its expression in Aspergillus niger and Aspergillus nidulans

• Published in: Current genetics Vol. 20; no. 4; pp. 293 - 299
• Main Authors: Kusters-van Someren, M.A. (Landbowhogeschool, Wageningen (Netherlands). Dept. of Genetics. Section of Molecular Genetics), Harmsen, J.A.M, Kester, H.C.M, Visser, J
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-09-1991; Berlin
• Subjects: Amino Acid Sequence; ASPERGILLUS NIDULANS; Aspergillus nidulans - enzymology; Aspergillus nidulans - genetics; ASPERGILLUS NIGER; Aspergillus niger - enzymology; Aspergillus niger - genetics; Base Sequence; Biological and medical sciences; Blotting, Southern; Blotting, Western; Chromatography, Affinity; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE; GENE EXPRESSION; GENES; Genes, Fungal; Genes. Genome; Heterologous expression; Introns; Laboratorium voor Erfelijkheidsleer; Laboratory of Genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; NUCLEOTIDE SEQUENCE; Pectin lyase; pelA gene; POLIGALACTURONASA; POLYGALACTURONASE; Polysaccharide-Lyases - genetics; predictions; Protein Biosynthesis; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Transformation, Genetic; Fungi; Purification; Pectin lyase; Nucleotide sequence; Enzyme; Aspergillus niger; Gene product; Chromosome DNA; Fungi Imperfecti; Molecular cloning; Gene expression; Thallophyta
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00318518

Aspergillus niger pectin lyases are encoded by a multigene family. The complete nucleotide sequence of the pectin lyase PLA-encoding gene pelA has been determined. Comparison of the deduced amino acid sequence with the deduced amino acid sequence of the other characterized pectin lyase, PLD, shows that the proteins share 69% amino acid identity. When grown on media with pectin as the sole carbon source, A. niger transformants containing multiple copies of the pelA gene show raised mRNA levels and overexpression of the gene product PLA compared with the wild-type strain. PLA was purified and characterized. In A. nidulans transformants PLA is also produced in medium containing a high concentration of glucose and no pectin.