Stir bar sorptive extraction: a new quantitative and comprehensive sampling technique for determination of chemical signal profiles from biological media

• Published in: Journal of chemical ecology Vol. 31; no. 2; pp. 377 - 392
• Main Authors: Soini, H.A, Bruce, K.E, Wiesler, D, David, F, Sandra, P, Novotny, M.V
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-02-2005; Springer Nature B.V
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Animals; Autoecology; Biological and medical sciences; Body Fluids - chemistry; Chemical ecology; Clinical Laboratory Techniques; Cricetinae; Ecology; Exocrine Glands - chemistry; extraction; Fundamental and applied biological sciences. Psychology; gas chromatography; Gas Chromatography-Mass Spectrometry; General aspects; hamsters; Mice; pheromones; Pheromones - analysis; Rats; Reproducibility of Results; Sampling techniques; Smell - physiology; urine; Urine - chemistry; Volatilization; Volatile compound; Urine; Chemical communication; gas chromatography-mass spectrometry; methods; Rodentia; Pheromone; chemical signals; Method; Pheromones; hamster urine; rat preputial gland; Gas chromatography; Vertebrata; Mammalia; Mouse; mouse urine; rat odor; Sampling; Odor; Mass spectrometry; stir bar sorptive extraction; Semiochemicals; volatiles
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/s10886-005-1347-8

Various applications of a novel sampling procedure in chemical ecology are outlined. The stir bar extraction method features the analytical reproducibility needed in recording the analytical profiles of volatile and semivolatile components of biological mixtures. This methodology has been demonstrated here through the examples of small volume urine samples, glandular tissue volatiles, and the air blown through animal cages. Its analytical merits are compared with those of the previously established purge-and-trap (dynamic headspace) technique.