The Drosophila melanogaster homolog of UBE3A is not imprinted in neurons

• Published in: Epigenetics Vol. 11; no. 9; pp. 637 - 642
• Main Authors: Hope, Kevin A, LeDoux, Mark S, Reiter, Lawrence T
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01-09-2016
• Subjects: Alleles; Animals; Brief Report; Drosophila melanogaster - genetics; Drosophila melanogaster - physiology; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Female; Genomic Imprinting; Male; Motor Activity - genetics; Neurons - metabolism; Polymorphism, Single Nucleotide; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; imprinting; SNPs; UBE3A; synaptic transmission; ERG; Drosophila melanogaster; DGRP; Climbing assay
• ISSN: 1559-2294; 1559-2308
• DOI: 10.1080/15592294.2016.1214783

In mammals, expression of UBE3A is epigenetically regulated in neurons and expression is restricted to the maternal copy of UBE3A. A recent report claimed that Drosophila melanogaster UBE3A homolog (Dube3a) is preferentially expressed from the maternal allele in fly brain, inferring an imprinting mechanism. However, complex epigenetic regulatory features of the mammalian imprinting center are not present in Drosophila, and allele specific expression of Dube3a has not been documented. We used behavioral and electrophysiological analysis of the Dube3a loss-of-function allele (Dube3a
) to investigate Dube3a imprinting in fly neurons. We found that motor impairment (climbing ability) and a newly-characterized defect in synaptic transmission are independent of parental inheritance of the Dube3a
allele. Furthermore, expression analysis of coding single nucleotide polymorphisms (SNPs) in Dube3a did not reveal allele specific expression differences among reciprocal crosses. These data indicate that Dube3a is neither imprinted nor preferentially expressed from the maternal allele in fly neurons.