Effects of temperature, storage period and the number of individuals on the detection of the false spider mite Cardinium endosymbiont

• Published in: Experimental & applied acarology Vol. 42; no. 1; pp. 17 - 21
• Main Authors: Novelli, Valdenice M, Freitas-Astúa, Juliana, Arrivabem, Fernanda, Locali-Fabris, Eliane C, Hilf, Mark E, Gottwald, Tim R, Machado, Marcos A
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Kluwer Academic Publishers 01-05-2007; Springer; Springer Nature B.V
• Subjects: Animals; Araneae; Biological and medical sciences; Brevipalpus phoenicis; Citrus leprosis virus; Cytophagaceae - genetics; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Generalities; Mites; Mites - microbiology; Phytopathology. Animal pests. Plant and forest protection; Polymerase Chain Reaction; Protozoa. Invertebrates; Sample Size; Sampling and conservation; Symbiosis; Temperature; Time Factors; Temperature; Methodology; Plant pathogen; Sample size; Citrus fruit; Rutaceae; Environmental factor; Brevipalpus phoenicis; Pest; Acari; Sampling and conservation; Dicotyledones; Angiospermae; Bacteria; Fruit tree; Vector; Tenuipalpidae; Duration; Symbiont; Citrus; Virus; Acariformes; Arachnida; Storage; Actinedida; Arthropoda; Spermatophyta; Invertebrata; Detection
• ISSN: 0168-8162; 1572-9702
• DOI: 10.1007/s10493-007-9070-5

Cardinium have been found as endosymbionts of Brevipalpus phoenicis, the mite vector of the Citrus leprosis virus. With the long-term objective being to understand the mechanisms of plant-virus-vector interactions, we evaluated the different storage conditions and periods, as well as the number of mites needed for PCR-amplification of such endosymbionts, making it possible to collect mites in different geographical regions without prolonged storage compromising subsequent analyses.