Effect of Sodium Selenite and Vitamin E on the Renal Cortex in Rats: An Ultrastructure Study

• Published in: Tissue & cell Vol. 46; no. 3; pp. 170 - 177
• Main Authors: Hussein, Adel M, Saleh, Hamid A, H.N., Mustafa
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01-06-2014
• Subjects: Advanced Basic Science; Animals; Bax protein; bcl-2-Associated X Protein - metabolism; Body Weight - physiology; Humans; Kidney - drug effects; Kidney - ultrastructure; Rats; Selenium - administration & dosage; Selenium - pharmacology; Sodium selenite; Sodium Selenite - toxicity; Ultrastructure; Vitamin E; Vitamin E - administration & dosage; Vitamin E - pharmacology; Sodium selenite; Bax protein; Vitamin E; Ultrastructure
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2014.03.002

Abstract This study examined the use of vitamin E to alleviate toxic effects of sodium selenite. Adult male albino rats ( n = 50) was divided into five groups. Group 1 was control, Groups 2 and 4 were treated with sodium selenite (2 mg/kg) for 2 and 4 weeks, respectively, Groups 3 and 5 were treated with sodium selenite (2 mg/kg) and vitamin E (100 mg/kg) for 2 and 4 weeks, respectively. Renal tissues were studied using anti-BCL2 and examined ultrastructurally. Positive Bax immunoreactivity was detected after 2 and more positive after 4 weeks and nearly all groups improved with co-administration of vitamin E. Ultrastructural study revealed lesions in Bowman's capsule and proximal convoluted tubules. The submicroscopic study revealed damage and necrosis of cortical structures after 2 and 4 weeks, respectively. After 4 weeks, cellular changes were seen, such as vacuolation and moderate degeneration of cells, widening of the urinary space scattered through the cortex with loss of cellular details, formation of apical buds, degeneration, and cellular rupture. Present findings disclosed an ameliorative effect of adding vitamin E to sodium selenite-induced changes in cortical tissues. Clinically, it is advised to add vitamin E to avoid selenium overdose hazards.