Hybrid photoacoustic and ultrasound section imaging with optical ultrasound detection

• Published in: Journal of biophotonics Vol. 6; no. 6-7; pp. 549 - 559
• Main Authors: Nuster, Robert, Schmitner, Nicole, Wurzinger, Gerhild, Gratt, Sibylle, Salvenmoser, Willi, Meyer, Dirk, Paltauf, Guenther
• Format: Journal Article
• Language: English
• Published: Berlin WILEY-VCH Verlag 01-06-2013; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: acoustic mirror; Animals; Female; Melanocytes - cytology; Multimodal Imaging - methods; optical detection; Optical Phenomena; optoacoustic imaging; photoacoustic imaging; Photoacoustic Techniques; section imaging; Ultrasonics; ultrasound imaging; Zebrafish
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.201200223

A setup is proposed that provides perfectly co‐registered photoacoustic (PA) and ultrasound (US) section images. Photoacoustic and ultrasound backscatter signals are generated by laser pulses coming from the same laser system, the latter by absorption of some of the laser energy on an optically absorbing target near the imaged object. By measuring both signals with the same optical detector, which is focused into the selected section by use of a cylindrical acoustic mirror, the information for both images is acquired simultaneously. Co‐registered PA and US images are obtained after applying the inverse Radon transform to the data, which are gathered while rotating the object relative to the detector. Phantom experiments demonstrate a resolution of 1.1 mm between the sections of both imaging modalities and a in‐plane resolution of about 60 µm and 120 µm for the US and PA modes, respectively. The complementary contrast mechanisms of the two modalities are shown by images of a zebrafish. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)