A novel chemical inducer of Streptococcus quorum sensing acts by inhibiting the pheromone-degrading endopeptidase PepO

• Published in: The Journal of biological chemistry Vol. 293; no. 3; pp. 931 - 940
• Main Authors: Pérez Morales, Tiara G., Ratia, Kiira, Wang, Duo-Sheng, Gogos, Artemis, Driver, Tom G., Federle, Michael J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19-01-2018; American Society for Biochemistry and Molecular Biology
• Subjects: antivirulence; Bacterial Proteins - metabolism; Endopeptidases - metabolism; Gene Expression Regulation, Bacterial; gene regulation; gram-positive bacteria; Gram-Positive Bacteria - metabolism; Microbiology; neprilysin; Neprilysin - metabolism; peptidase; pheromone; Pheromones - metabolism; quorum quenching; quorum sensing; Quorum Sensing - physiology; Signal Transduction - genetics; Signal Transduction - physiology; Streptococcus pyogenes - genetics; Streptococcus pyogenes - metabolism; gram-positive bacteria; quorum sensing; neprilysin; gene regulation; peptidase; quorum quenching; pheromone; antivirulence
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M117.810994

Bacteria produce chemical signals (pheromones) to coordinate behaviors across a population in a process termed quorum sensing (QS). QS systems comprising peptide pheromones and their corresponding Rgg receptors are widespread among Firmicutes and may be useful targets for manipulating microbial behaviors, like suppressing virulence. The Rgg2/3 QS circuit of the human PATHogen Streptococcus pyogenes controls genes affecting resistance to host lysozyme in response to short hydrophobic pheromones (SHPs). Considering that artificial activation of a QS PATHway may be as useful in the objective of manipulating bacteria as inhibiting it, we sought to identify small-molecule inducers of the Rgg2/3 QS system. We report the identification of a small molecule, P516-0475, that specifically induced expression of Rgg2/3-regulated genes in the presence of SHP pheromones at concentrations lower than typically required for QS induction. In searching for the mode of action of P516-0475, we discovered that an S. pyogenes mutant deficient in pepO, a neprilysin-like metalloendopeptidase that degrades SHP pheromones, was unresponsive to the compound. P516-0475 directly inhibited recombinant PepO in vitro as an uncompetitive inhibitor. We conclude that this compound induces QS by stabilizing SHP pheromones in culture. Our study indicates the usefulness of cell-based screens that modulate PATHway activities to identify unanticipated therapeutic targets contributing to QS signaling.