Identification of aminobenzoic acids as selective inhibitors of the N-terminal phosphatase of soluble epoxide hydrolase

• Published in: Bioscience, biotechnology, and biochemistry Vol. 87; no. 5; pp. 511 - 515
• Main Authors: Kihara, Yasuhiro, Nishimura, Erina, Kanai, Chisato, Kitano, Yoshikazu, Suzuki, Eriko, Hasumi, Keiji
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 24-04-2023
• Subjects: Aminobenzoates; Enzyme Inhibitors - pharmacology; Epoxide Hydrolases - metabolism; Phosphoric Monoester Hydrolases; enzyme inhibition; soluble epoxide hydrolase; phosphatase
• ISSN: 1347-6947; 1347-6947
• DOI: 10.1093/bbb/zbad016

ABSTRACT
Soluble epoxide hydrolase (EC 3.3.2.10) is a key enzyme in the regulation of inflammation and metabolism, whereas, the role of its N-terminal phosphatase activity (N-phos) has been poorly understood because of a lack of selective inhibitors. Here we report 4-aminobenzoic (Ki 15.3 µm) and 3-amino-4-hydroxy benzoic acid (Ki 11.7 µm) as selective competitive inhibitors of N-phos.