Chromosome Localization of Highly Repetitive Human DNA's and Amplified Ribosomal DNA with Restriction Enzymes

Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the...

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Bibliographic Details
Published in:	Science (American Association for the Advancement of Science) Vol. 219; no. 4583; pp. 395 - 397
Main Authors:	Miller, Dorothy A., Choi, Ye-Chin, Miller, Orlando J.
Format:	Journal Article
Language:	English
Published:	United States The American Association for the Advancement of Science 28-01-1983 American Association for the Advancement of Science
Subjects:	C band Chromosome Banding Chromosome Mapping Chromosomes Delta cells DNA DNA Restriction Enzymes Enzymes Gene Amplification Genes Human chromosomes Humans Repetitive Sequences, Nucleic Acid Ribosomal DNA RNA, Ribosomal - genetics rRNA genes Satellite DNA
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Summary:	Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.6294832