Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor

• Published in: Sensors (Basel, Switzerland) Vol. 7; no. 5; pp. 719 - 729
• Main Authors: Seo, Kang-Deuk, Lee, Kwang-Pill, Gopalan, Anantha Iyengar, Chung, Sang J., Lim, Yong Taik, Choi, Seong-Ho
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 31-05-2007; Molecular Diversity Preservation International (MDPI)
• Subjects: Aqueous solutions; Biosensors; Communication; Copolymers; Electrodes; Enzymes; Gamma rays; Gold; Hydrogen peroxide; Nanoparticles; Polymerization; Radiation; Sensors; South Korea
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s7050719

Enzyme modified electrodes were fabricated with poly(aniline-co-m-aminophenol). Electrochemical polymerization of aniline and m-aminophenol wasperformed to get the film of copolymer on the surface of gold electrode. Modifiedelectrodes were fabricated by two methods, physical entrapment and covalent cross-linking.In one of the method, gold nanoparticles were loaded into the copolymer film andhorseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymerfilm through physical entrapment. In the other method, the amino and -OH groups in thecopolymer are utilized to form covalent functionalization with HRP via glutaric dialdehydeas cross-linker/mediator. The conducting copolymer/enzyme modified electrodes preparedby physical entrapment/covalent functionalization of enzyme were tested forelectrocatalytic activities towards sensing of H2O2. Amperometric results indicate thatenzyme modified electrode via physical entrapment possesses better electrocatalyticperformance over covalent functionalized enzyme electrode.