Rodent IgE-binding factor genes are members of an endogenous, retrovirus-like gene family

• Published in: The Journal of immunology (1950) Vol. 136; no. 11; pp. 4283 - 4290
• Main Authors: Moore, KW, Jardieu, P, Mietz, JA, Trounstine, ML, Kuff, EL, Ishizaka, K, Martens, CL
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01-06-1986; American Association of Immunologists
• Subjects: Absorption; Amino Acid Sequence; Analysis of the immune response. Humoral and cellular immunity; Animals; Antibodies, Viral - analysis; Antibody Specificity; Antigens, Viral - genetics; Antigens, Viral - immunology; Biological and medical sciences; Cloning, Molecular; DNA - metabolism; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Genes; Genes, Intracisternal A-Particle; Genes, Viral; Immunobiology; Lymphokines - genetics; Lymphokines - metabolism; Lymphokines, interleukins ( function, expression); Mice; Mice, Inbred BALB C; Nucleic Acid Hybridization; Peptides - immunology; Prostatic Secretory Proteins; Proto-Oncogenes; Rats; Rats, Inbred Lew; Regulatory factors and their cellular receptors; Retroviridae - genetics; Allergy; Immunoglobulins; Immune response; Soluble factor; IgE; Rodentia; Retroviridae; Biosynthesis; B-Lymphocyte; Lymphokine; Virus; Vertebrata; Regulation(control); Mammalia; Complementary DNA; Gene; Molecular cloning
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.136.11.4283

Synthesis of IgE by B lymphocytes can be regulated by soluble lymphocyte factors which have affinity for the Fc region of IgE (IgE-binding factors). In previous studies, we identified cDNA clones encoding rodent IgE-binding factors by direct expression in transfected mammalian cells. Here we show that IgE-binding factor cDNA clone 8.3 is a member of the endogenous, retrovirus-like intracisternal A-particle gene family of the mouse. This conclusion is supported by blot hybridization, DNA sequence comparisons, heteroduplex analysis, and immunochemical cross-reactivity of the encoded proteins. The results identify a member of this highly reiterated gene family with a role in regulation of the allergic immune response.