An Arabidopsis thaliana protein interacts with a movement protein of Turnip crinkle virus in yeast cells and in vitro

• Published in: Journal of general virology Vol. 82; no. 5; pp. 1245 - 1251
• Main Authors: Lin, Bing, Heaton, Louis A
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-05-2001
• Subjects: Amino Acid Sequence; Ap8 protein; Arabidopsis; Arabidopsis Proteins; Arabidopsis thaliana; Atp8 protein; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular Sequence Data; Mutagenesis; Oligopeptides - metabolism; Phenotype; Plant Proteins - metabolism; Plant Viral Movement Proteins; Polymorphism, Restriction Fragment Length; Saccharomyces cerevisiae; Tombusviridae - metabolism; Turnip crinkle virus; Viral Proteins - metabolism
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-82-5-1245

Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502, USA 1
Author for correspondence: Louis Heaton. Fax [phone]. e-mail heaton{at}plantpath.ksu.edu
Plant virus movement proteins bind host components to promote virus movement from initially infected cells to neighbouring cells. In this study, cDNA clones encoding p8 and p9, two small proteins required for the movement of Turnip crinkle virus , were used as ‘bait’ in a yeast two-hybrid system to screen an Arabidopsis thaliana cDNA library for interactive proteins. One A . thaliana clone was identified that encodes a protein, designated Atp8, which interacted with p8 in yeast cells and in vitro . The apparent full-length of Atp8 mRNA was sequenced and shown to encode a protein with two possible transmembrane helices, several potential phosphorylation sites and two ‘RGD’ sequences.