Porins from Salmonella enterica serovar typhimurium induce TNF-α, IL-6 and IL-8 release by CD14-independent and CD11a/CD18-dependent mechanisms

• Published in: Microbiology (Society for General Microbiology) Vol. 147; no. Pt 10; pp. 2697 - 2704
• Main Authors: GALDIERO, Marilena, D'ISANTO, Marina, VITIELLO, Mariateresa, FINAMORE, Emiliana, PELUSO, Lucia, GALDIERO, Massimiliano
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01-10-2001
• Subjects: Bacterial diseases; Bacteriology; Biological and medical sciences; CD18 Antigens - metabolism; Cell Line; Cytokines - metabolism; Experimental bacterial diseases and models; Fundamental and applied biological sciences. Psychology; Humans; Infectious diseases; Interleukin-1 - metabolism; Interleukin-8 - metabolism; Lipopolysaccharide Receptors - metabolism; Lipopolysaccharides - immunology; Lymphocyte Function-Associated Antigen-1 - metabolism; Medical sciences; Microbiology; Monocytes - immunology; Porins - physiology; Receptors, Cell Surface - metabolism; Salmonella enterica; Salmonella typhimurium - immunology; Salmonella typhimurium - physiology; Serotyping; Tumor Necrosis Factor-alpha - metabolism; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Porin; Steroid hormone; Cytokine; Salmonella typhimurium; Biological activity; Infection; Interleukin 6; Vitamin D; Gram positive bacteria; Tumor necrosis factor; Bacteriosis; Lipopolysaccharide; Bacteria; Salmonellosis; Component; Tumor necrosis factor α; Interleukin 8; Release; Enterobacteriaceae; Gram negative bacteria
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-147-10-2697

Lipopolysaccharide (LPS) of Gram-negative bacteria and several surface components of Gram-positive bacteria utilize CD14 and CD11a/18 as cellular receptors to induce expression and release of cytokines. Of the surface components of Gram-negative bacteria, porins exhibit a biological activity similar to that of LPS. The results in this paper show that the mechanism of stimulation by porins of THP-1 cells enriched in CD14 receptor after treatment with 1,25-dihydroxyvitamin D(3) (vitamin D(3)) is independent of this receptor, but is partially dependent on CD11a/18 integrins.