Limited diffusibility of gene products directed by a single nucleus in the cytoplasm of multinucleated myofibres

• Published in: FEBS letters Vol. 337; no. 1; pp. 18 - 22
• Main Authors: Ono, Tomoko, Ono, Katsuhiko, Mizukawa, Kiminao, Ohta, Tetsuya, Tsuchiya, Tomofusa, Tsuda, Masaaki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03-01-1994; Elsevier
• Subjects: Animals; beta-Galactosidase - genetics; beta-Galactosidase - metabolism; Biological and medical sciences; Cell Nucleus - enzymology; Cytoplasm - metabolism; Diffusion; Diseases of striated muscles. Neuromuscular diseases; DNA - metabolism; Duchenne's muscular dystrophy; Dystrophin - genetics; Gene therapy; Gene transfer; Gene Transfer Techniques; Genetic Therapy; Kinetics; Luciferases - genetics; Medical sciences; Mice; Mice, Inbred ICR; Microscopy, Electron; Muscles - enzymology; Muscles - ultrastructure; Muscular Dystrophies - therapy; Myofibre; Neurology; Plasmids; Subcellular domain; β-Galactosidase; Myofibre; Subcellular domain; Gene transfer; Gene therapy; Duchenne's muscular dystrophy; β-Galactosidase; Embryonic development; Animal model; Neuromuscular diseases; Nervous system diseases; Enzyme; Rodentia; Intramuscular administration; Genetic disease; Vertebrata; Mammalia; Gene; Mouse; Glycosidases; Duchenne muscular dystrophy; Hydrolases; Diffusing capacity; O-Glycosidases; Cytoplasm
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(94)80621-7

Two types of β-galactosidase genes, whose products are distributed in the nucleus (Nα-gal) or cytoplasm (Cβ-gal), were injected with fructose intramuscularly into the quadriceps of adult mice. Regionally restricted and overlapped distributions of both gene products were observed in the myofibres. These findings indicate that Nβ-gal is incorporated into the nucleus responsible for its synthesis and that Cβ-gal becomes located in the vicinity of the nucleus after its synthesis. This restricted location of C β-gal in myofibres remained unchanged during the development of infant mouse muscle. Thus, the gene products directed by the nucleus of myofibres seem to show limited diffusibility, suggesting a universal localization of subcellular domains in myofibres.