The Role of TSHR, PTEN and RASSF1A Promoters' Methylation Status for Non-Invasive Detection of Papillary Thyroid Carcinoma

• Published in: Journal of clinical medicine Vol. 11; no. 16; p. 4917
• Main Authors: Klimaitė, Raimonda, Kazokaitė, Mintautė, Kondrotienė, Aistė, Daukšienė, Dalia, Sabaliauskaitė, Rasa, Žukauskaitė, Kristina, Žilaitienė, Birutė, Jarmalaitė, Sonata, Daukša, Albertas
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21-08-2022; MDPI
• Subjects: Clinical medicine; Disease; DNA methylation; Gene expression; Medical prognosis; Pathogenesis; Plasma; Surgery; Thyroid cancer; Tumors; United States > US; Germany; PTEN; papillary thyroid carcinoma; RASSF1A; methylation; PTC; TSHR
• ISSN: 2077-0383; 2077-0383
• DOI: 10.3390/jcm11164917

Aim: We investigated whether a difference exists between TSHR, PTEN and RASSF1A methylation status in plasma of subjects with papillary thyroid cancer (PTC). Methods: Peripheral blood samples were collected from 68 patients with PTC and 86 healthy controls (HC). Thyroid cancer tissue and corresponding adjacent normal tissue methylation levels were analyzed. DNA methylation level changes in TSHR, PTEN and RASSF1A genes were analyzed by quantitative methylation-sensitive polymerase chain reaction. Results: We observed that the methylation level of TSHR was significantly higher in the thyroid cancer tissue compared to adjacent normal tissue (p = 0.040). TSHR methylation levels in the PTC group plasma samples were significantly higher compared to HC (p = 0.022). After surgery, PTC plasma samples showed lower TSHR and PTEN methylation levels compared to the levels before surgery (p = 0.003, p = 0.031, respectively). The TSHR methylation level was significantly higher in PTC with larger tumor size (>2 cm) (p < 0.001), and lymph node metastases (p = 0.01), lymphovascular invasion (p = 0.02) and multifocality (p = 0.013) 0ROC analysis revealed that the TSHR methylation level provides high accuracy in distinguishing PTC from HC (p = 0.022, AUC of 0.616). Conclusion: TSHR methylation in peripheral blood samples is expected to be a sensitive and specific minimally invasive tool for the diagnosis of PTC, especially in combination with other diagnostic means.