Investigating A Multi-Domain Polyketide Synthase in Amphidinium carterae

• Published in: Marine drugs Vol. 21; no. 8; p. 425
• Main Authors: Haq, Saddef, Oyler, Benjamin L., Williams, Ernest, Khan, Mohd M., Goodlett, David R., Bachvaroff, Tsvetan, Place, Allen R.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 27-07-2023; MDPI
• Subjects: Acetates; Acetic acid; Algae; Algal blooms; Amphidinium carterae; Biosynthesis; Cerulenin; Chromatography; Covalent bonds; Dinoflagellata; dinoflagellate; Dinoflagellates; Dissociation; Eutrophication; Fatty acids; Lipids; Mass spectrometry; Mass spectroscopy; Microorganisms; Peptides; PKS; Polyketide synthase; Post-transcription; Proteins; Segregation; Synthesis; Synthetic peptides; toxin; Toxins; Western blotting
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21080425

Dinoflagellates are unicellular organisms that are implicated in harmful algal blooms (HABs) caused by potent toxins that are produced through polyketide synthase (PKS) pathways. However, the exact mechanisms of toxin synthesis are unknown due to a lack of genomic segregation of fat, toxins, and other PKS-based pathways. To better understand the underlying mechanisms, the actions and expression of the PKS proteins were investigated using the toxic dinoflagellate Amphidinium carterae as a model. Cerulenin, a known ketosynthase inhibitor, was shown to reduce acetate incorporation into all fat classes with the toxins amphidinol and sulpho-amphidinol. The mass spectrometry analysis of cerulenin-reacted synthetic peptides derived from ketosynthase domains of A. carterae multimodular PKS transcripts demonstrated a strong covalent bond that could be localized using collision-induced dissociation. One multi-modular PKS sequence present in all dinoflagellates surveyed to date was found to lack an AT domain in toxin-producing species, indicating trans-acting domains, and was shown by Western blotting to be post-transcriptionally processed. These results demonstrate how toxin synthesis in dinoflagellates can be differentiated from fat synthesis despite common underlying pathway.