Identification of quantitative trait loci (QTLs) regulating leaf SPAD value and trichome density in mungbean ( Vigna radiata L.) using genotyping-by-sequencing (GBS) approach

• Published in: PeerJ (San Francisco, CA) Vol. 12; p. e16722
• Main Authors: Kumari, Nikki, Mishra, Gyan Prakash, Dikshit, Harsh Kumar, Gupta, Soma, Roy, Anirban, Sinha, Subodh Kumar, Mishra, Dwijesh C, Das, Shouvik, Kumar, Ranjeet R, Nair, Ramakrishnan Madhavan, Aski, Muraleedhar
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 21-02-2024; PeerJ, Inc; PeerJ Inc
• Subjects: Agricultural production; Agricultural research; Analysis; Beans; Candidate genes; Chlorophyll; Chlorophyll content; Chromosome 7; Chromosome Mapping; Disease resistance; Gene mapping; Genes; Genotype; Genotypes; Genotyping; Green gram; Inbreeding; Leaves; Localization; Marker assisted selection; Methionine; Plant Leaves - genetics; Polymorphism; Protein binding; Proteins; Quantitative genetics; Quantitative trait loci; Quantitative Trait Loci - genetics; Soil; Soybeans; Transcription factors; Trichomes; Trichomes - genetics; Vigna - genetics; India; Green gram; Trichomes; Chlorophyll content; Candidate genes; Marker assisted selection
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.16722

Quantitative trait loci (QTL) mapping is used for the precise localization of genomic regions regulating various traits in plants. Two major QTLs regulating Soil Plant Analysis Development (SPAD) value (
) and trichome density (
) in mungbean were identified using recombinant inbred line (RIL) populations (PMR-1×Pusa Baisakhi) on chromosome 7. Functional analysis of QTL region identified 35 candidate genes for SPAD value (16 No) and trichome (19 No) traits. The candidate genes regulating trichome density on the dorsal leaf surface of the mungbean include
, and
which encodes for ZFP6, TFs bHLH DNA-binding superfamily protein, and MYB102, respectively. Also, candidate genes having vital roles in chlorophyll biosynthesis are
, and
, which encodes for s-adenosyl-L-methionine, FTSHI1 protein, and CRS2-associated factor, respectively. The findings unfolded the opportunity for the development of customized genotypes having high SPAD value and high trichome density having a possible role in yield and mungbean yellow vein mosaic India virus (MYMIV) resistance in mungbean.