Effective Aeromonas specific monoclonal antibody for immunodiagnosis

• Published in: Asian Pacific journal of tropical biomedicine Vol. 8; no. 1; pp. 7 - 13
• Main Authors: Mahakunkijcharoen, Yuvadee, Hirunpetcharat, Chakrit, Malijunbua, Sunisa, Muangkaew, Watcharamat, Paksanont, Suporn
• Format: Journal Article
• Language: English
• Published: Medknow Publications and Media Pvt. Ltd 01-01-2018; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Thailand%Department of Microbiology, Faculty of Public Health, Mahidol University, Thailand%Department of Medical Technology, Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Thailand; Wolters Kluwer Medknow Publications
• Subjects: aeromonas; Antibodies; Antigens; Bacteria; Enzymes; Gastroenteritis; Gastrointestinal system; gastrointestinal tract; Health aspects; immunodiagnosis; Infection; Monoclonal antibodies; monoclonal antibody; Technology; Water; Wounds; Gastrointestinal tract; Monoclonal antibody; Immunodiagnosis; Aeromonas
• ISSN: 2221-1691
• DOI: 10.4103/2221-1691.221128

Objective:To identify the monoclonal antibody specific to Aeromonas spp.,a Gram negative bacteria causing gastroenteritis and wound infection.Methods:The monoclone,namely 88F2-3F4,was produced from hybridoma technology.The specificity of antibody secreted from 88F2-3F4 was tested against other Gram negative bacteria frequently found in gastrointestinal tract.Then the antibody was used for searching Aeromonas antigens in artificial seeded rectal swab cultures by dot-blot enzyme linked immunosorbent assay.Results:88F2-3F4 produced an antibody that recognized an antigen with a molecular mass of 8.5 kDa in all 123 isolates of the seven Aeromonas species tested,but recognized no epitope of any other Gram-negative bacterium typically found in the gastrointestinal tract.A dot-blot enzyme linked immunosorbent assay based on this antibody showed 86.49％ sensitivity and 92.13％ specificity.Conclusions:88F2-3F4 monoclonal antibody could react with all Aeromonas isolates,but not other Gram negative bacteria,therefore it should be a useful tool for the detection of Aeromonas antigen in clinical and environmental samples.