Involvement of PAF in postallergic propranolol-induced bronchoconstriction in guinea-pigs

• Published in: The European respiratory journal Vol. 9; no. 10; pp. 2064 - 2069
• Main Authors: Fujimura, M, Tsujiura, M, Songur, N, Myou, S, Matsuda, T
• Format: Journal Article
• Language: English
• Published: Leeds Eur Respiratory Soc 01-10-1996; Maney
• Subjects: Administration, Inhalation; Adrenergic beta-Antagonists - administration & dosage; Adrenergic beta-Antagonists - adverse effects; Anesthesia, General; Animals; Antigens; Asthma - physiopathology; Azepines - administration & dosage; Azepines - pharmacology; Biological and medical sciences; Bronchoconstriction - drug effects; Chronic obstructive pulmonary disease, asthma; Disease Models, Animal; Dose-Response Relationship, Drug; Guinea Pigs; Hypersensitivity - physiopathology; Immunization; Inflammation Mediators - physiology; Injections, Intravenous; Male; Medical sciences; Platelet Activating Factor - antagonists & inhibitors; Platelet Activating Factor - physiology; Pneumology; Propranolol - administration & dosage; Propranolol - adverse effects; Respiration, Artificial; Time Factors; Triazoles - administration & dosage; Triazoles - pharmacology; Induced bronchoconstriction; Vertebrata; Mammalia; Guinea pig; Pathophysiology; Respiratory disease; Animal; Rodentia; Exploration; Platelet activating factor; Beta blocking agent
• ISSN: 0903-1936; 1399-3003
• DOI: 10.1183/09031936.96.09102064

Administration of propranolol can provoke bronchoconstriction in asthmatic patients. Recently, we successfully developed a guinea-pig model for propranolol-induced bronchoconstriction (PIB). We hypothesized that such bronchoconstriction may result from the inflammatory mediators released by an allergic reaction. The purpose of this study was to examine the role of platelet-activating factor (PAF) in the development of PIB after allergic reaction. Propranolol, at a concentration of 10 mg.mL-1 was inhaled 20 min after antigen challenge in passively sensitized, anaesthetized and artificially-ventilated guinea-pigs. The animals were treated intravenously with PAF antagonists, E6123 (1 and 10 micrograms.kg-1) or Y-24180 (1 and 10 mg.kg-1), 10 min before or 15 min after antigen challenge. Propranolol inhaled 20 min after antigen challenge caused bronchoconstriction. E6123 and Y-24180 administered 15 min after antigen challenge as well as 10 min before antigen challenge reduced the PIB in a dose-dependent manner. We conclude that platelet-activating factor may contribute to the development of propranolol-induced bronchoconstriction after allergic reaction in our guinea-pig model.