Gene regulation of the Lactobacillus vini in response to industrial stress in the fuel ethanol production

• Published in: Microbiological research Vol. 236; p. 126450
• Main Authors: Mendonça, Allyson Andrade, da Silva, Paula Katharina Nogueira, Calazans, Tiago Luiz Santana, de Souza, Rafael Barros, Elsztein, Carolina, de Morais Junior, Marcos Antonio
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01-06-2020
• Subjects: Acid stress; Adaptation, Physiological - genetics; Cell adaptation; Ethanol; Fermentation; Gene Expression Regulation, Bacterial; Genome, Bacterial; Hydrogen-Ion Concentration; Industrial fermentation; Industrial Microbiology; Lactobacillus - genetics; Lactobacillus - growth & development; Lactobacillus - metabolism; Regulatory modules; Stress, Physiological - genetics; Temperature; Thermotolerance; Transcriptome; USP genes; Industrial fermentation; USP genes; Thermotolerance; Acid stress; Regulatory modules; Cell adaptation
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2020.126450

•Lactobacillus vini is tolerant to most of industrial stress agents.•Ethanol acts as sensibilization agent for Lactobacillus vini cells.•Different forms of stress share common biological damages.•uspII is a general stress response gene in Lactobacillus vini.•usp genes define regulatory modules responding to different stressing agents.
The industrial ethanol fermentation imposes several stresses to microorganisms. However, some bacterial species are well adapted and manage to endure these harmful conditions. Lactobacillus vini is one of the most found bacteria in these environments, indicating the existence of efficient tolerance mechanisms. In view of this premise, the present study aimed to describe the tolerance of L. vini to several stressing agents encounter in industrial environments and the genetic components of the stress response. In general, L. vini showed significant tolerance to stressors commonly found in fuel-ethanol fermentations, and only doses higher than normally reached in processes restrained its growth. The lag phase and the growth rate were the most responsive kinetic parameter affected. Gene expression analysis revealed that uspII gene positively responded to all conditions tested, a typical profile of a general stress response gene. In addition, the results also revealed aspects of regulatory modules of co-expressed genes responding to different stresses, and also the similarities of response mechanism with basis in common cellular damages. Altogether, these data contribute to uncover the factors that could favour L. vini in the industrial fermentation which could be shared with other well adapted species and reports the first stress response genes in this bacterium.