Lipid droplets in skeletal muscle during grass snake (Natrix natrix L.) development

• Published in: Biochimica et biophysica acta. Molecular and cell biology of lipids Vol. 1867; no. 2; p. 159086
• Main Authors: Dubińska-Magiera, Magda, Lewandowski, Damian, Cysewski, Dominik, Pawlak, Seweryn, Najbar, Bartłomiej, Daczewska, Małgorzata
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-02-2022
• Subjects: Animals; Colubridae - growth & development; Colubridae - metabolism; Lipid droplet; Lipid Droplets - metabolism; Lipid Metabolism; Muscle development; Muscle, Skeletal - growth & development; Muscle, Skeletal - metabolism; Perilipins; Proteome - analysis; Proteome - metabolism; Reptile; Skeletal muscle; Muscle development; Perilipins; Reptile; Lipid droplet; Skeletal muscle
• ISSN: 1388-1981; 1879-2618; 1879-2618
• DOI: 10.1016/j.bbalip.2021.159086

Lipid droplets (LDs) are common organelles observed in Eucaryota. They are multifunctional organelles (involved in lipid storage, metabolism, and trafficking) that originate from endoplasmic reticulum (ER). LDs consist of a neutral lipid core, made up of diacyl- and triacylglycerols (DAGs and TAGs) and cholesterol esters (CEs), surrounded by a phospholipid monolayer and proteins, which are necessary for their structure and dynamics.
Here, we report the protein and lipid composition as well as characterization and dynamics of grass snake (Natrix natrix) skeletal muscle LDs at different developmental stages. In the present study, we used detailed morphometric, LC-MS, quantitative lipidomic analyses of LDs isolated from the skeletal muscles of the snake embryos, immunofluorescence, and TEM.
Our study also provides a valuable insight concerning the LDs' multifunctionality and ability to interact with a variety of organelles. These LD features are reflected in their proteome composition, which contains scaffold proteins, metabolic enzymes signalling polypeptides, proteins necessary for the formation of docking sites, and many others. We also provide insights into the biogenesis and growth of muscle LDs goes beyond the conventional mechanism based on the synthesis and incorporation of TAGs and LD fusion. We assume that the formation and functioning of grass snake muscle LDs are based on additional mechanisms that have not yet been identified, which could be related to the unique features of reptiles that are manifested in the after-hatching period of life, such as a reptile-specific strategy for energy saving during hibernation.
•The LDs multifunctionality is reflected in their proteome composition•The synthesis and incorporation of TAGs and LDs fusion are the grass snake skeletal muscles LDs growth mechanisms•The formation and functioning of grass snake muscle LDs are based on additional, not identified yet mechanisms